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Fourteen paired human cadaveric lower legs were mounted in a loading frame. Computed tomography scans were obtained in unloaded state (75\u00a0N) and single-leg loaded stand (700\u00a0N) of each specimen in five foot positions: neutral, 15\u00b0 external rotation, 15\u00b0 internal rotation, 20\u00b0 dorsiflexion, and 20\u00b0 plantarflexion. An automated three-dimensional measurement protocol was used to assess clear space (diastasis), translational angle (rotation), and vertical offset (fibular shortening) in each foot position and loading condition. Foot positioning had a significant effect on DTFJ\u00a0configuration. Largest effects were related to clear space increase by 0.46\u00a0mm (SD 0.21\u00a0mm) in loaded dorsal flexion and translation angle of 2.36\u00b0 (SD 1.03\u00b0) in loaded external rotation, both versus loaded neutral position. Loading had no effect on clear space and vertical offset in any position. Translation angle was significantly influenced under loading by \u2212\u20090.81\u00b0 (SD 0.69\u00b0) in internal rotation only. Foot positioning noticeably influences the measurements when evaluating DTFJ\u00a0configuration. Loading seems to have no relevant effect on native ankles in neutral position.<\/jats:p>","DOI":"10.1038\/s41598-022-12963-2","type":"journal-article","created":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T09:04:11Z","timestamp":1654592651000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Effect of weightbearing and foot positioning on 3D distal tibiofibular joint parameters"],"prefix":"10.1038","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1087-7503","authenticated-orcid":false,"given":"Firas","family":"Souleiman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5611-7476","authenticated-orcid":false,"given":"Martin","family":"Heilemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9936-5979","authenticated-orcid":false,"given":"Robert","family":"Hennings","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6510-2943","authenticated-orcid":false,"given":"Pierre","family":"Hepp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9795-115X","authenticated-orcid":false,"given":"Boyko","family":"Gueorguiev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7778-2480","authenticated-orcid":false,"given":"Geoff","family":"Richards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5051-0998","authenticated-orcid":false,"given":"Georg","family":"Osterhoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7148-9569","authenticated-orcid":false,"given":"Dominic","family":"Gehweiler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,6,7]]},"reference":[{"key":"12963_CR1","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1007\/s00068-014-0466-8","volume":"41","author":"S Rammelt","year":"2015","unstructured":"Rammelt, S. & Obruba, P. 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