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The biochemical and histopathological alterations associated with thioacetamide (TAA)-induced liver fibrosis in rats were evaluated in vivo where rats received the two extracts at three different dose levels (100, 200 and 400\u00a0mg\/kg p.o, daily) for 15 consecutive days with induction of hepatotoxicity by TAA (200\u00a0mg\/kg\/day, i.p.) at 14th and 15th days. Results of the present study showed a significant restoration in liver function biomarkers viz<jats:italic>.<\/jats:italic> alanine transaminase (ALT), aspartate transaminase (AST), gamma glutamyl transferase and total bilirubin. The liver homogenates exhibited increased levels of antioxidant biomarkers: reduced glutathione (GSH) and catalase (CAT), accompanied with decline in malondialdehyde (MDA). Furthermore, treated groups exhibited a significant suppression in liver inflammatory cytokines: tumor necrosis factor-\u03b1 (TNF-\u03b1) and interlukin-6 (IL-6), and fibrotic biomarker: alpha smooth muscle relaxant. Histopathological examination of the liver showed normality of hepatocytes. Noteworthy, <jats:italic>P. indica<\/jats:italic> extract showed better hepatoprotective activity than <jats:italic>P. auriculata<\/jats:italic>, particularly at 200\u00a0mg\/kg. To sum up, all these results indicated the hepatoprotective properties of both extracts, as well as their antifibrotic effect was evidenced by reduction in hepatic collagen deposition. However, additional experiments are required to isolate their individual secondary metabolites, assess the toxicity of the extracts and explore the involved mechanism of action.<\/jats:p>","DOI":"10.1038\/s41598-022-13718-9","type":"journal-article","created":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T11:03:51Z","timestamp":1655204631000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Phytochemical profiling and anti-fibrotic activities of Plumbago indica L. and Plumbago auriculata Lam. in thioacetamide-induced liver fibrosis in rats"],"prefix":"10.1038","volume":"12","author":[{"given":"Nabil Mohamed","family":"Selim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mina Michael","family":"Melk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farouk Rasmy","family":"Melek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalia Osama","family":"Saleh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mansour","family":"Sobeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seham S.","family":"El-Hawary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,6,14]]},"reference":[{"key":"13718_CR1","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1159\/000374096","volume":"33","author":"M Pinzani","year":"2015","unstructured":"Pinzani, M. 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