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In this study, a biomimetic chip that simulates the microenvironment of the implant and bone in vitro was developed (tissue-on-chip of group T and group C) to study the correlation of cell movement velocity (CMV), direction (CMD), acceleration (CMA), and cell attachment number (CA) with the surface micromorphology of the Titanium material. Computational fluid dynamics (CFD) was used for flow analysis. Changes in intraosseous pressure (IOP), local blood perfusion index (LBPI), new bone microstructure, microvessel density (MVD), and bone-implant contact (BIC) in beagle dogs were detected as implant surface alterations. Surface skewness (<jats:italic>Ssk<\/jats:italic>) and surface arithmetic mean height (<jats:italic>Sa<\/jats:italic>) were the most important negative factors for high CMV, accounting for 51% and 32%, respectively, of all the influencing factors. Higher <jats:italic>Ssk<\/jats:italic> (<jats:italic>Ssk<\/jats:italic><jats:sub><jats:italic>T<\/jats:italic><\/jats:sub>\u2009&gt;\u20090, <jats:italic>Ssk<\/jats:italic><jats:sub><jats:italic>C<\/jats:italic><\/jats:sub>\u2009&lt;\u20090) and <jats:italic>Sa<\/jats:italic> (<jats:italic>Sa<\/jats:italic><jats:sub><jats:italic>T<\/jats:italic><\/jats:sub>\u2009&gt;\u2009<jats:italic>Sa<\/jats:italic><jats:sub><jats:italic>C<\/jats:italic><\/jats:sub>) resulted in lower CMV (CMV<jats:sub>T<\/jats:sub>:CMV<jats:sub><jats:italic>C<\/jats:italic><\/jats:sub>\u2009=\u20090.41:1), greater CA (CA<jats:sub>T<\/jats:sub>:CA<jats:sub>C<\/jats:sub>\u2009=\u20091.44:1), and higher BIC (BIC<jats:sub>T<\/jats:sub>:BIC<jats:sub>C<\/jats:sub>\u2009=\u20093.06:1) (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). The surface micromorphology influenced the CMD of MG-63 cells within 20\u00a0\u03bcm from the material surface. 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