{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T02:06:39Z","timestamp":1793239599776,"version":"4.5.0"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004001","name":"Science and Technology Foundation of Guizhou Province","doi-asserted-by":"crossref","award":["[2019]1194"],"award-info":[{"award-number":["[2019]1194"]}],"id":[{"id":"10.13039\/501100004001","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Science and Technology Foundation of Guizhou Provincial Health Commission","award":["gzwjkj2019-1-107"],"award-info":[{"award-number":["gzwjkj2019-1-107"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960139"],"award-info":[{"award-number":["81960139"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004001","name":"Guizhou Science and Technology Department","doi-asserted-by":"crossref","award":["QKHPTRC2018-5636-2"],"award-info":[{"award-number":["QKHPTRC2018-5636-2"]}],"id":[{"id":"10.13039\/501100004001","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004001","name":"Guizhou Science and Technology Department","doi-asserted-by":"crossref","award":["QKHPTRC2020-2201"],"award-info":[{"award-number":["QKHPTRC2020-2201"]}],"id":[{"id":"10.13039\/501100004001","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004001","name":"Guizhou Science and Technology Department","doi-asserted-by":"crossref","award":["QKHJC2020-1Y304"],"award-info":[{"award-number":["QKHJC2020-1Y304"]}],"id":[{"id":"10.13039\/501100004001","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Kidney functions, including electrolyte and water reabsorption and secretion, could be influenced by circulating hormones. The pituitary gland produces a variety of hormones and cytokines; however, the influence of these factors on the kidney has not been well explained and explored. To provide more in-depth information and insights to support the pituitary\u2013kidney axis connection, we used mouse pituitary and kidney single-cell transcriptomics data from the GEO database for further analysis. Based on a ligand\u2013receptor pair analysis, cell\u2013cell interaction patterns between the pituitary and kidney cell types were described. Key ligand\u2013receptor pairs, such as GH-GHR, PTN-SDC2, PTN-SDC4, and DLK1-NOTCH3, were relatively active in the pituitary\u2013kidney axis. These ligand\u2013receptor pairs mainly target proximal tubule cells, principal cells, the loop of Henle, intercalated cells, pericytes, mesangial cells, and fibroblasts, and these cells are related to physiological processes, such as substance reabsorption, angiogenesis, and tissue repair. Our results suggested that the pituitary gland might directly regulate kidney function by secreting multiple hormones or cytokines and indicated that the above ligand\u2013receptor pairs might represent a new research focus for studies on kidney function or kidney disease.<\/jats:p>","DOI":"10.1038\/s41598-022-14680-2","type":"journal-article","created":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T05:03:09Z","timestamp":1656651789000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Single-cell RNA sequencing data analysis suggests the cell\u2013cell interaction patterns of the pituitary\u2013kidney axis"],"prefix":"10.1038","volume":"12","author":[{"given":"Yiyao","family":"Deng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Da","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaomin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,7,1]]},"reference":[{"issue":"12","key":"14680_CR1","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1038\/nrneph.2015.131","volume":"11","author":"R Lu","year":"2015","unstructured":"Lu, R., Kiernan, M. C., Murray, A., Rosner, M. H. & Ronco, C. Kidney-brain crosstalk in the acute and chronic setting. Nat. Rev. Nephrol. 11(12), 707\u2013719. https:\/\/doi.org\/10.1038\/nrneph.2015.131 (2015).","journal-title":"Nat. Rev. Nephrol."},{"issue":"3","key":"14680_CR2","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1016\/j.kint.2019.10.032","volume":"97","author":"S Tanaka","year":"2020","unstructured":"Tanaka, S. & Okusa, M. D. Crosstalk between the nervous system and the kidney. Kidney Int. 97(3), 466\u2013476. https:\/\/doi.org\/10.1016\/j.kint.2019.10.032 (2020).","journal-title":"Kidney Int."},{"issue":"3","key":"14680_CR3","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1186\/cc13907","volume":"18","author":"A Nongnuch","year":"2014","unstructured":"Nongnuch, A., Panorchan, K. & Davenport, A. Brain-kidney crosstalk. Critical Care (London, England) 18(3), 225. https:\/\/doi.org\/10.1186\/cc13907 (2014).","journal-title":"Critical Care (London, England)"},{"issue":"9","key":"14680_CR4","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1038\/s41581-019-0161-4","volume":"15","author":"J Drube","year":"2019","unstructured":"Drube, J. et al. Clinical practice recommendations for growth hormone treatment in children with chronic kidney disease. Nat. Rev. Nephrol. 15(9), 577\u2013589. https:\/\/doi.org\/10.1038\/s41581-019-0161-4 (2019).","journal-title":"Nat. Rev. Nephrol."},{"issue":"3","key":"14680_CR5","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1681\/asn.2019070677","volume":"31","author":"DE Wesson","year":"2020","unstructured":"Wesson, D. E., Buysse, J. M. & Bushinsky, D. A. Mechanisms of metabolic acidosis-induced kidney injury in chronic kidney disease. J. Am. Soc. Nephrol. 31(3), 469\u2013482. https:\/\/doi.org\/10.1681\/asn.2019070677 (2020).","journal-title":"J. Am. Soc. Nephrol."},{"issue":"4","key":"14680_CR6","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1038\/nrendo.2013.38","volume":"9","author":"JD Veldhuis","year":"2013","unstructured":"Veldhuis, J. D. Changes in pituitary function with ageing and implications for patient care. Nat. Rev. Endocrinol. 9(4), 205\u2013215. https:\/\/doi.org\/10.1038\/nrendo.2013.38 (2013).","journal-title":"Nat. Rev. Endocrinol."},{"issue":"1","key":"14680_CR7","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1186\/s12916-019-1332-7","volume":"17","author":"AL Conroy","year":"2019","unstructured":"Conroy, A. L. et al. Acute kidney injury is associated with impaired cognition and chronic kidney disease in a prospective cohort of children with severe malaria. BMC Med. 17(1), 98. https:\/\/doi.org\/10.1186\/s12916-019-1332-7 (2019).","journal-title":"BMC Med."},{"issue":"7","key":"14680_CR8","doi-asserted-by":"publisher","first-page":"2127","DOI":"10.1681\/asn.2005010005","volume":"16","author":"M Kurella","year":"2005","unstructured":"Kurella, M. et al. Chronic kidney disease and cognitive impairment in the elderly: The health, aging, and body composition study. J. Am. Soc. Nephrol. 16(7), 2127\u20132133. https:\/\/doi.org\/10.1681\/asn.2005010005 (2005).","journal-title":"J. Am. Soc. Nephrol."},{"key":"14680_CR9","doi-asserted-by":"publisher","DOI":"10.1161\/jaha.114.000933","author":"VC Wu","year":"2014","unstructured":"Wu, V. C. et al. The impact of acute kidney injury on the long-term risk of stroke. J. Am. Heart Assoc. https:\/\/doi.org\/10.1161\/jaha.114.000933 (2014).","journal-title":"J. Am. Heart Assoc."},{"issue":"10","key":"14680_CR10","doi-asserted-by":"publisher","first-page":"942","DOI":"10.1038\/ki.2011.241","volume":"81","author":"ME Grams","year":"2012","unstructured":"Grams, M. E. & Rabb, H. The distant organ effects of acute kidney injury. Kidney Int. 81(10), 942\u2013948. https:\/\/doi.org\/10.1038\/ki.2011.241 (2012).","journal-title":"Kidney Int."},{"issue":"7","key":"14680_CR11","doi-asserted-by":"publisher","first-page":"1888","DOI":"10.1016\/j.cell.2019.05.031","volume":"177","author":"T Stuart","year":"2019","unstructured":"Stuart, T. et al. Comprehensive integration of single-cell data. Cell 177(7), 1888-1902.e1821. https:\/\/doi.org\/10.1016\/j.cell.2019.05.031 (2019).","journal-title":"Cell"},{"issue":"12","key":"14680_CR12","doi-asserted-by":"publisher","first-page":"1289","DOI":"10.1038\/s41592-019-0619-0","volume":"16","author":"I Korsunsky","year":"2019","unstructured":"Korsunsky, I. et al. Fast, sensitive and accurate integration of single-cell data with Harmony. Nat. Methods 16(12), 1289\u20131296. https:\/\/doi.org\/10.1038\/s41592-019-0619-0 (2019).","journal-title":"Nat. Methods"},{"issue":"1","key":"14680_CR13","doi-asserted-by":"publisher","first-page":"1088","DOI":"10.1038\/s41467-021-21246-9","volume":"12","author":"S Jin","year":"2021","unstructured":"Jin, S. et al. Inference and analysis of cell-cell communication using CellChat. Nat. Commun. 12(1), 1088. https:\/\/doi.org\/10.1038\/s41467-021-21246-9 (2021).","journal-title":"Nat. Commun."},{"issue":"D1","key":"14680_CR14","doi-asserted-by":"publisher","first-page":"D605","DOI":"10.1093\/nar\/gkaa1074","volume":"49","author":"D Szklarczyk","year":"2021","unstructured":"Szklarczyk, D. et al. The STRING database in 2021: Customizable protein\u2013protein networks, and functional characterization of user-uploaded gene\/measurement sets. Nucleic Acids Res. 49(D1), D605-d612. https:\/\/doi.org\/10.1093\/nar\/gkaa1074 (2021).","journal-title":"Nucleic Acids Res."},{"issue":"6220","key":"14680_CR15","doi-asserted-by":"publisher","first-page":"1260419","DOI":"10.1126\/science.1260419","volume":"347","author":"M Uhl\u00e9n","year":"2015","unstructured":"Uhl\u00e9n, M. et al. Proteomics. Tissue-based map of the human proteome. Science 347(6220), 1260419. https:\/\/doi.org\/10.1126\/science.1260419 (2015).","journal-title":"Science"},{"issue":"8","key":"14680_CR16","doi-asserted-by":"publisher","first-page":"2511","DOI":"10.1007\/s00467-021-05097-6","volume":"36","author":"D Haffner","year":"2021","unstructured":"Haffner, D., Grund, A. & Leifheit-Nestler, M. Renal effects of growth hormone in health and in kidney disease. Pediatr. Nephrol. 36(8), 2511\u20132530. https:\/\/doi.org\/10.1007\/s00467-021-05097-6 (2021).","journal-title":"Pediatr. Nephrol."},{"issue":"6","key":"14680_CR17","doi-asserted-by":"publisher","first-page":"3061","DOI":"10.1210\/endo.131.6.1446640","volume":"131","author":"E Chin","year":"1992","unstructured":"Chin, E., Zhou, J. & Bondy, C. A. Renal growth hormone receptor gene expression: Relationship to renal insulin-like growth factor system. Endocrinology 131(6), 3061\u20133066. https:\/\/doi.org\/10.1210\/endo.131.6.1446640 (1992).","journal-title":"Endocrinology"},{"issue":"2","key":"14680_CR18","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1007\/bf02990065","volume":"3","author":"HC Mertani","year":"1995","unstructured":"Mertani, H. C., Delehaye-Zervas, M. C., Martini, J. F., Postel-Vinay, M. C. & Morel, G. Localization of growth hormone receptor messenger RNA in human tissues. Endocrine 3(2), 135\u2013142. https:\/\/doi.org\/10.1007\/bf02990065 (1995).","journal-title":"Endocrine"},{"issue":"5","key":"14680_CR19","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.1681\/ASN.2013050563","volume":"25","author":"A Scarpellini","year":"2014","unstructured":"Scarpellini, A. et al. Syndecan-4 knockout leads to reduced extracellular transglutaminase-2 and protects against tubulointerstitial fibrosis. J. Am. Soc. Nephrol. 25(5), 1013\u20131027. https:\/\/doi.org\/10.1681\/ASN.2013050563 (2014).","journal-title":"J. Am. Soc. Nephrol."},{"key":"14680_CR20","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.cytogfr.2019.03.006","volume":"46","author":"G Traustad\u00f3ttir","year":"2019","unstructured":"Traustad\u00f3ttir, G. et al. The imprinted gene Delta like non-canonical Notch ligand 1 (Dlk1) is conserved in mammals, and serves a growth modulatory role during tissue development and regeneration through Notch dependent and independent mechanisms. Cytokine Growth Factor Rev. 46, 17\u201327. https:\/\/doi.org\/10.1016\/j.cytogfr.2019.03.006 (2019).","journal-title":"Cytokine Growth Factor Rev."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-022-14680-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-022-14680-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-022-14680-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T01:27:59Z","timestamp":1669339679000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-022-14680-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,1]]},"references-count":20,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["14680"],"URL":"https:\/\/doi.org\/10.1038\/s41598-022-14680-2","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,1]]},"assertion":[{"value":"27 April 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 June 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 July 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"11147"}}