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Immunopathology believes that abnormal T cell function and excessive production of autoantibodies by B cells are involved in multi-organ damage. Human umbilical cord mesenchymal stem cells (hUCMSCs) therapies have endowed with promise in SLE, while the function of MSC-derived extracellular vesicles (MSC-EVs) was still unclear. Extracellular vesicles (EVs) are subcellular components secreted by a paracellular mechanism and are essentially a group of nanoparticles. EVs play a vital role in cell-to-cell communication by acting as biological transporters. New evidence has shown beneficial effects of MSC-EVs on autoimmune diseases, such as their immunomodulatory properties. In this study, we investigated whether hUCMSCs derived extracellular vesicles (hUCMSC-EVs) could regulate abnormal immune responses of T cells or B cells in SLE. We isolated splenic mononuclear cells from MRL\/lpr mice, a classical animal model of SLE. PBS (Phosphate-buffered saline), 2\u2009\u00d7\u200910<jats:sup>5<\/jats:sup>hUCMSCs, 25\u00a0\u00b5g\/ml hUCMSC-EVs, 50\u00a0\u00b5g\/ml hUCMSC-EVs were co-cultured with 2\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>activated splenic mononuclear cells for 3\u00a0days in vitro, respectively. The proportions of CD4<jats:sup>+<\/jats:sup>T cell subsets, B cells and the concentrations of cytokines were detected. Both hUCMSCs and hUCMSC-EVs inhibited CD4<jats:sup>+<\/jats:sup>T cells, increased the production of T helper (Th)17 cells, promoted the production of interleukin (IL)-17 and transforming growth factor beta1 (TGF-\u03b21) (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), although they had no significant effects on Th1, Th2, T follicular helper (Tfh), regulatory T (Treg) cells and IL-10 (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05); only hUCMSCs inhibited CD19<jats:sup>+<\/jats:sup>B cells, promoted the production of interferon-gamma (IFN-\u03b3) and IL-4 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). hUCMSCs exert immunoregulatory effects on SLE at least partially through hUCMSC-EVs in vitro, therefore, hUCMSC-EVs play novel and potential regulator roles in SLE.<\/jats:p>","DOI":"10.1038\/s41598-022-17331-8","type":"journal-article","created":{"date-parts":[[2022,7,30]],"date-time":"2022-07-30T10:03:12Z","timestamp":1659175392000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Human umbilical cord mesenchymal stem cells derived extracellular vesicles regulate acquired immune response of lupus mouse in vitro"],"prefix":"10.1038","volume":"12","author":[{"given":"Min","family":"Xie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuifang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"She","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifeng","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jueyi","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marady","family":"Hun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Senlin","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuqing","family":"Wan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jidong","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,7,30]]},"reference":[{"issue":"6","key":"17331_CR1","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1038\/cmi.2008.52","volume":"5","author":"K Zhou","year":"2008","unstructured":"Zhou, K. et al. 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