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In this prospective cross-sectional study, healthy volunteers attending the same workplace environment underwent a noninvasive ocular surface workup by means of Keratograph 5\u00a0M (Oculus, Wetzlar, Germany) in the same day at 2 different time points: (i) in the early morning before wearing face mask (T0); (ii) after 8\u00a0h of continuous face mask use (T1). Noninvasive break-up time (NIBUT), tear meniscus height (TMH), ocular redness and meibomian gland dropout were measured. All subjects were asked to complete the Ocular Surface Disease Index (OSDI) questionnaire before and after 8\u00a0h of face mask wearing. Data from 20 healthy subjects (10 males and 10 females, mean age 25.1\u2009\u00b1\u20093.9\u00a0years) were included. Mean value of TMH decreased significantly from 0.29\u2009\u00b1\u20090.07 at T0 to 0.23\u2009\u00b1\u20090.07\u00a0mm at T1 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001); conversely, mean values of NIBUT, redness score and meibomian gland dropout did not change significantly after continuous face mask wearing (always<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.532). Concerning ocular discomfort symptoms, mean value of OSDI score worsened significantly at T1 compared to T0 (from 12.9\u2009\u00b1\u200912.6 to 19.4\u2009\u00b1\u200912.0;<jats:italic>P<\/jats:italic>\u2009=\u20090.017). Continuous face mask wearing for 8\u00a0h led to decreased TMH associated with the onset of ocular discomfort symptoms in young healthy subjects.<\/jats:p>","DOI":"10.1038\/s41598-022-17486-4","type":"journal-article","created":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T10:04:36Z","timestamp":1659089076000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Diurnal changes of noninvasive parameters of ocular surface in healthy subjects before and after continuous face mask wearing during the COVID-19 pandemic"],"prefix":"10.1038","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2617-0289","authenticated-orcid":false,"given":"Giuseppe","family":"Giannaccare","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Pellegrini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimiliano","family":"Borselli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlotta","family":"Senni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Bruno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Scorcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,7,29]]},"reference":[{"key":"17486_CR1","doi-asserted-by":"publisher","first-page":"e148","DOI":"10.1016\/S2589-7500(20)30293-4","volume":"3","author":"B Rader","year":"2021","unstructured":"Rader, B. et al. 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