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Our attempt here was to investigate the influence elicited by hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) on functional changes of MECs. Our study showed that cell viability of MECs was inhibited after cells were exposed to oxidative agents. Cells subjected to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> at the concentration of 150\u00a0\u00b5M for 24\u00a0h and 48\u00a0h exhibited an elevation of reactive oxygen species (ROS) activity, decrease of total antioxidant capacity (T-AOC) level and reduced mitochondrial membrane potential (\u0394\u03a8m) compared with control cells. 95 protein spots with more than twofold difference were detected in two dimensional electrophoresis (2DE) gels through proteomics assay following H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> exposure for 48\u00a0h, 10 proteins were identified through TOF\/MS analysis. Among the proteomic changes explored, 8 proteins related to energy metabolism, mitochondrial\u00a0function, structural regulation, and cell cycle control were downregulated. 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