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To understand the safety, efficacy, and mechanism of action of cell-based therapies, it is imperative to follow their biodistribution noninvasively. Positron-emission-tomography (PET)-based non-invasive imaging of cell trafficking offers such a potential. Herein, we evaluated and compared three different ready-to-use direct cell radiolabeling synthons, [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-DFO-Bn-NCS, [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-Hy\n                    <jats:sub>3<\/jats:sub>\n                    ADA\n                    <jats:sup>5<\/jats:sup>\n                    -NCS, and [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-Hy\n                    <jats:sub>3<\/jats:sub>\n                    ADA\n                    <jats:sup>5<\/jats:sup>\n                    -SA for PET imaging-based trafficking of white blood cells (WBCs) and stem cells (SCs) up to 7\u00a0days in athymic nude mice. We compared the degree of\n                    <jats:sup>89<\/jats:sup>\n                    Zr complexation and percentage of cell radiolabeling efficiencies with each. All three synthons, [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-DFO-Bn-NCS, [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-Hy\n                    <jats:sub>3<\/jats:sub>\n                    ADA\n                    <jats:sup>5<\/jats:sup>\n                    -NCS, and [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-Hy\n                    <jats:sub>3<\/jats:sub>\n                    ADA\n                    <jats:sup>5<\/jats:sup>\n                    -SA, were successfully prepared, and used for radiolabeling of WBCs and SCs. The highest cell radiolabeling yield was found for [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-DFO-Bn-NCS, followed by [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-Hy\n                    <jats:sub>3<\/jats:sub>\n                    ADA\n                    <jats:sup>5<\/jats:sup>\n                    -NCS, and [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-Hy\n                    <jats:sub>3<\/jats:sub>\n                    ADA\n                    <jats:sup>5<\/jats:sup>\n                    -SA. In terms of biodistribution, WBCs radiolabeled with [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-DFO-Bn-NCS or [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-Hy\n                    <jats:sub>3<\/jats:sub>\n                    ADA\n                    <jats:sup>5<\/jats:sup>\n                    -NCS, were primarily accumulated in liver and spleen, whereas SCs radiolabeled with [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-DFO-Bn-NCS or [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-Hy\n                    <jats:sub>3<\/jats:sub>\n                    ADA\n                    <jats:sup>5<\/jats:sup>\n                    -NCS were found in lung, liver and spleen. A high bone uptake was observed for both WBCs and SCs radiolabeled with [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-Hy\n                    <jats:sub>3<\/jats:sub>\n                    ADA\n                    <jats:sup>5<\/jats:sup>\n                    -SA, suggesting in-vivo instability of [\n                    <jats:sup>89<\/jats:sup>\n                    Zr]Zr-Hy\n                    <jats:sub>3<\/jats:sub>\n                    ADA\n                    <jats:sup>5<\/jats:sup>\n                    -SA synthon. This study offers an appropriate selection of ready-to-use radiolabeling synthons for noninvasive trafficking of WBCs, SCs and other cell-based therapies.\n                  <\/jats:p>","DOI":"10.1038\/s41598-022-19953-4","type":"journal-article","created":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T05:03:05Z","timestamp":1663563785000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of different 89Zr-labeled synthons for direct labeling and tracking of white blood cells and stem cells in healthy athymic mice"],"prefix":"10.1038","volume":"12","author":[{"given":"Aditya","family":"Bansal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shalini","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedikt","family":"Klasen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"R\u00f6sch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mukesh K.","family":"Pandey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,9,19]]},"reference":[{"key":"19953_CR1","doi-asserted-by":"publisher","first-page":"3908","DOI":"10.1096\/fj.08-112896","volume":"22","author":"YW Noh","year":"2008","unstructured":"Noh, Y. 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