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Here, we elucidated the pathogenome of a global collection of <jats:italic>E. coli<\/jats:italic> ST1485 isolates from diverse sources retrieved from public databases and a high-quality sequenced complete genome of colistin-resistant <jats:italic>E. coli<\/jats:italic> strain CFSAN061771 isolated from raw milk cheese which designated as a reference strain. CFSAN061771 belongs to O83:H42-ST1485 pathotype and carries a conjugative ColV plasmid, pCFSAN061771_01, combining extraintestinal virulence genes (<jats:italic>ompt<\/jats:italic>, <jats:italic>sitA<\/jats:italic>, <jats:italic>iroN<\/jats:italic>, <jats:italic>etsC<\/jats:italic>, <jats:italic>traT<\/jats:italic>, <jats:italic>cvaC<\/jats:italic>, <jats:italic>hylF<\/jats:italic>, <jats:italic>iss<\/jats:italic>, <jats:italic>tsh<\/jats:italic>, <jats:italic>mchf<\/jats:italic>, <jats:italic>iucC<\/jats:italic>, <jats:italic>iutA<\/jats:italic>) with a multidrug resistance island (<jats:italic>bla<\/jats:italic><jats:sub>TEM-1<\/jats:sub>, <jats:italic>aph<\/jats:italic>(<jats:italic>6<\/jats:italic>)<jats:italic>-Id<\/jats:italic>, <jats:italic>aph<\/jats:italic>(<jats:italic>3\u2033<\/jats:italic>)<jats:italic>-Ib<\/jats:italic>, <jats:italic>sul2<\/jats:italic>, <jats:italic>dfrA14<\/jats:italic>). Comparative genomic analysis revealed a high frequency of pCFSAN061771_01-like plasmids in <jats:italic>E. coli<\/jats:italic> ST1485. A notable evolutionary genetic event in <jats:italic>E. coli<\/jats:italic> ST1485 strains is the acquisition of a pCFSAN061771_02-like plasmid, which confers resistance to several antimicrobials, tellurium, and quaternary ammonium compounds. The identical virulence and antibiotic resistance profiles identified in some human and animal strains are worrisome. This is the first study to emphasize the significance of <jats:italic>E. coli<\/jats:italic> ST1485 as a global high-risk virulent and multidrug-resistant clone with zoonotic potential.<\/jats:p>","DOI":"10.1038\/s41598-022-20342-0","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T13:06:55Z","timestamp":1667394415000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Pathogenome comparison and global phylogeny of Escherichia coli ST1485 strains"],"prefix":"10.1038","volume":"12","author":[{"given":"Ahmed M.","family":"Hammad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narjol","family":"Gonzalez-Escalona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira","family":"El Tahan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasser H.","family":"Abbas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara S.K.","family":"Koenig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Allu\u00e9-Guardia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Eppinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,2]]},"reference":[{"key":"20342_CR1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00135-18","author":"AR Manges","year":"2019","unstructured":"Manges, A. 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