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This study investigated the acute effects of two cannabis terpenes, \u03b2-caryophyllene and \u03b1-pinene, on zebrafish locomotion, anxiety-like, and boldness behaviour using the open field exploration and novel object approach tests. \u03b2-caryophyllene was administered in 0.02%, 0.2%, 2.0%, and 4% doses. \u03b1<jats:italic>-<\/jats:italic>pinene was administered in 0.01%, 0.02%, and 0.1% doses. As \u03b1-pinene is a racemic compound, we also tested its (+) and (\u2212) enantiomers to observe any differential effects. \u03b2-caryophyllene had only a sedative effect at the highest dose tested. \u03b1-pinene had differing dose-dependent effects on anxiety-like and motor variables. Specifically, (+)-\u03b1-pinene and (\u2212)-\u03b1-pinene had significant effects on anxiety measures, time spent in the thigmotaxis (outer) or center zone, in the open field test, as well as locomotor variables, swimming velocity and immobility. (+\u2009\/\u2212)-\u03b1-pinene showed only a small effect on the open field test on immobility at the 0.1% dose. This study demonstrates that \u03b1-pinene can have a sedative or anxiolytic effect in zebrafish and may have different medicinal properties when isolated into its (+) or (\u2212) enantiomers.<\/jats:p>","DOI":"10.1038\/s41598-022-21552-2","type":"journal-article","created":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T14:15:41Z","timestamp":1665756941000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Effects of super-class cannabis terpenes beta-caryophyllene and alpha-pinene on zebrafish behavioural biomarkers"],"prefix":"10.1038","volume":"12","author":[{"given":"Andr\u00e9a","family":"Johnson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alycia","family":"Stewart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismaeel","family":"El-Hakim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trevor J.","family":"Hamilton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,10,14]]},"reference":[{"issue":"7","key":"21552_CR1","doi-asserted-by":"publisher","first-page":"1344","DOI":"10.1111\/j.1476-5381.2011.01238.x","volume":"163","author":"EB Russo","year":"2011","unstructured":"Russo, E. 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