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Here, a combination of in vitro tests was performed on 33\n                    <jats:italic>Bifidobacterium<\/jats:italic>\n                    strains as probiotic candidates for IBS. In addition to the classical tests performed, the detection of the serine protease inhibitor (serpin) enzyme capable of decreasing the high proteolytic activity found in IBS patients was included. Three serpin-positive strains were selected:\n                    <jats:italic>Bifidobacterium breve<\/jats:italic>\n                    CNCM I-5644,\n                    <jats:italic>Bifidobacterium longum<\/jats:italic>\n                    subsp.\n                    <jats:italic>infantis<\/jats:italic>\n                    CNCM I-5645 and\n                    <jats:italic>B. longum<\/jats:italic>\n                    CNCM I-5646 for their immunomodulation properties and protection of intestinal epithelial integrity in vitro. Furthermore, we found that\n                    <jats:italic>B. breve<\/jats:italic>\n                    CNCM I-5644 strain prevented intestinal hyperpermeability by upregulating\n                    <jats:italic>Cingulin<\/jats:italic>\n                    and\n                    <jats:italic>Tight Junction Protein<\/jats:italic>\n                    1 mRNA levels and reducing pro-inflammatory markers. The ability of CNCM I-5644 strain to restore intestinal hyperpermeability (FITC-dextran) was shown in the murine model of low-grade inflammation induced by dinitrobenzene sulfonic acid (DNBS). This effect of this strain was corroborated in a second model of IBS, the neonatal maternal separation model in mice. Altogether, these data suggest that serpin-positive\n                    <jats:italic>B. breve<\/jats:italic>\n                    CNCM I-5644 may partially prevent disorders associated with increased barrier permeability such as IBS.\n                  <\/jats:p>","DOI":"10.1038\/s41598-022-21746-8","type":"journal-article","created":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T08:04:14Z","timestamp":1668672254000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Serpin-positive Bifidobacterium breve CNCM I-5644 improves intestinal permeability in two models of irritable bowel syndrome"],"prefix":"10.1038","volume":"12","author":[{"given":"Edgar","family":"Torres-Maravilla","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Holowacz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanne","family":"Delannoy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lo\u00efc","family":"Lenoir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsa","family":"Jacouton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandie","family":"Gervason","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u00ebva","family":"Meynier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Sophie","family":"Boucard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric A.","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Barbut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis G.","family":"Berm\u00fadez-Humar\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Langella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Judith","family":"Waligora-Dupriet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,17]]},"reference":[{"key":"21746_CR1","doi-asserted-by":"publisher","first-page":"17","DOI":"10.14309\/ajg.0000000000001036","volume":"116","author":"BE Lacy","year":"2021","unstructured":"Lacy, B. 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