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This study investigated its prevalence and its STs\/alleles, performed phylogenetic analysis, and considered the distribution of risk factors associated with\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp. infections in West Ismailia, Ismailia governorate. Sociodemographic data, exposure factors, and previous parasitic infection status were recorded for symptomatic and asymptomatic individuals. Microscopy, polymerase chain reaction, sequencing, and phylogenetic analysis for\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp. isolated from fecal samples were performed. Eighty\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp.-infected individuals (15.3%) were examined. The age of the individuals ranged between 0.60 and 85.0 (mean 17.10\u2009\u00b1\u200915.70), the male\/female ratio was 33\/47, and the asymptomatic\/symptomatic ratio was 55\/25. The findings demonstrate clear evidence of direct contact with animals, poor water quality, and previous parasitic infections. Eleven samples yielded three\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    STs (ST1: allele 4, ST2: alleles 9 and 12, and ST3: allele 34), with ST3 (45.5%) representing the most common subtype. Phylogenetic analysis with a robust bootstrap revealed three distinct clades for isolates of each subtype. This study updates the epidemiological knowledge of the distribution of\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp. STs in Egypt and expands the current understanding of the prevalence, risk factor frequencies, and genetic diversity of this protist in the studied area.\n                  <\/jats:p>","DOI":"10.1038\/s41598-022-23360-0","type":"journal-article","created":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T09:03:19Z","timestamp":1667984599000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Subtypes and phylogenetic analysis of Blastocystis sp. isolates from West Ismailia, Egypt"],"prefix":"10.1038","volume":"12","author":[{"given":"Shahira A.","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heba S.","family":"El-Mahallawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samar Farag","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina","family":"Angelici","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyriacos","family":"Hasapis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taisir","family":"Saber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panagiotis","family":"Karanis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,9]]},"reference":[{"key":"23360_CR1","doi-asserted-by":"publisher","unstructured":"Ahmed, S. 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