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Here, we describe an efficient one-step labeling procedure of [\n                    <jats:sup>18<\/jats:sup>\n                    F]SFB starting from a tin precursor. This method circumvents volatile radioactive side-products and simplifies automatization. [\n                    <jats:sup>18<\/jats:sup>\n                    F]SFB was obtained after HPLC purification in a yield of 42\u2009+\u20094% and a radiochemical purity (RCP)\u2009&gt;\u200999% (n\u2009=\u20096). In addition, we investigate the automation of the coupling of [\n                    <jats:sup>18<\/jats:sup>\n                    F]SFB to a nanobody (cAbBcII10, targeting \u03b2-lactamase enzyme) and purification by size exclusion chromatography (PD-10 desalting column) to remove unconjugated reagent. Production and use of [\n                    <jats:sup>18<\/jats:sup>\n                    F]SFB were implemented on a radiosynthesis unit (Neptis\n                    <jats:sup>\u00ae<\/jats:sup>\n                    ). The fully automated radiosynthesis process including purification and formulation required 160\u00a0min of synthesis time. [\n                    <jats:sup>18<\/jats:sup>\n                    F]SFB-labeled nanobody was obtained in a yield of 21\u2009+\u20092% (activity yield 12\u2009+\u20091% non-decay corrected) and a radiochemical purity (RCP) of\u2009&gt;\u200995% (n\u2009=\u20093). This approach simplifies [\n                    <jats:sup>18<\/jats:sup>\n                    F]SFB synthesis to one-step, enhances the yield in comparison to the previous report and enables the production of radiolabeled nanobody on the same synthesis module.\n                  <\/jats:p>","DOI":"10.1038\/s41598-022-23552-8","type":"journal-article","created":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T07:27:21Z","timestamp":1667546841000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Fully automated 18F-fluorination of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB) for indirect labelling of nanobodies"],"prefix":"10.1038","volume":"12","author":[{"given":"Surasa","family":"Nagachinta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Novelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoann","family":"Joyard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Maindron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Riss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvestre","family":"Dammicco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,4]]},"reference":[{"issue":"6","key":"23552_CR1","doi-asserted-by":"publisher","first-page":"991","DOI":"10.2967\/jnumed.108.060418","volume":"50","author":"WJ McBride","year":"2009","unstructured":"McBride, W. 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