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MicroRNA expression abnormalities are possibly disease-specific and may contribute to GDM pathological processes. To date, there is limited data on miRNA profiling in GDM, especially that involves a longitudinal study. Here, we performed miRNA expression profiling in the entire duration of pregnancy (during pregnancy until parturition and postpartum) using a miRNA- polymerase chain reaction array (miRNA-PCRArray) and <jats:italic>in-silico<\/jats:italic> analysis to identify unique miRNAs expression and their anticipated target genes in Malay maternal serum. MiRNA expression levels and their unique potential as biomarkers were explored in this work. In GDM patients, the expression levels of <jats:italic>hsa-miR-193a<\/jats:italic>, <jats:italic>hsa-miR-21, hsa-miR-23a<\/jats:italic>, and <jats:italic>hsa-miR-361<\/jats:italic> were significantly increased, but <jats:italic>miR-130a<\/jats:italic> was significantly downregulated. The area under the curve (AUC) and receiver operating characteristic (ROC) curve study demonstrated that <jats:italic>hsa-miR-193a<\/jats:italic> (AUC\u2009=\u20090.89060\u2009\u00b1\u200904,470, <jats:italic>P<\/jats:italic>\u2009=\u20090.0001), <jats:italic>hsa-miR-21<\/jats:italic> (AUC\u2009=\u20090.89500\u2009\u00b1\u200904,411, <jats:italic>P<\/jats:italic>\u2009=\u20090.0001), <jats:italic>and miR-130a<\/jats:italic> (AUC\u2009=\u20090.6939\u2009\u00b1\u20090.05845, <jats:italic>P<\/jats:italic>\u2009=\u20090.0025) had potential biomarker features in GDM. <jats:italic>In-silico<\/jats:italic> analysis also revealed that <jats:italic>KLF<\/jats:italic> (Kruppel-Like family of transcription factor), <jats:italic>ZNF25<\/jats:italic> (Zinc finger protein 25), <jats:italic>AFF4<\/jats:italic> (ALF transcription elongation factor 4), <jats:italic>C1orf143<\/jats:italic> (long intergenic non-protein coding RNA 2869), <jats:italic>SRSF2<\/jats:italic> (serine and arginine rich splicing factor 2), and <jats:italic>ZNF655<\/jats:italic> (Zinc finger protein 655) were prominent genes targeted by the common nodes of miR23a, miR130, miR193a, miR21, and miR361.Our findings suggest that circulating microRNAs in the first trimester has the potential for GDM screening in the Malay population.<\/jats:p>","DOI":"10.1038\/s41598-022-23816-3","type":"journal-article","created":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T19:57:28Z","timestamp":1669406248000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Analysis of serum circulating MicroRNAs level in Malaysian patients with gestational diabetes mellitus"],"prefix":"10.1038","volume":"12","author":[{"given":"Sajad","family":"Jamalpour","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shamsul Mohd","family":"Zain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza","family":"Vazifehmand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahurin","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuh Fen","family":"Pung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hesam","family":"Kamyab","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siti Zawiah","family":"Omar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,11,24]]},"reference":[{"key":"23816_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2393-10-7","volume":"10","author":"O Grissa","year":"2010","unstructured":"Grissa, O. et al. 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