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Their production usually requires pathogenic isolation hosts. We identified a novel strain, <jats:italic>Escherichia coli<\/jats:italic> ST155, that could serve as a production host for three different polyvalent phages (\u03d5Ph_SE03, \u03d5Ph_SD01, and \u03d5Ph_EC01), thus superseding the use of individual isolation hosts. Upon propagation in <jats:italic>E. coli<\/jats:italic> ST155, the phages demonstrated differential intergeneric infectivity against <jats:italic>Salmonella enterica<\/jats:italic>, <jats:italic>E. coli<\/jats:italic> OP50<jats:italic>, Shigella dysenteriae<\/jats:italic>, <jats:italic>E. coli<\/jats:italic> MDR<jats:italic>,<\/jats:italic> and <jats:italic>Acinetobacter baumannii<\/jats:italic>. Phages were characterised based on morphology, latent period, burst size, the efficiency of plating, and restriction enzyme profile. Survival assay on <jats:italic>Caenorhabditis elegans,<\/jats:italic> the absence of Shiga toxin, and enterotoxigenic <jats:italic>E. coli<\/jats:italic> virulence genes indicated that <jats:italic>E. coli<\/jats:italic> ST155 could be non-pathogenic. Lack of antibiotic resistance and absence of functional prophages rendered the host suitable for environmental applications. As a proof-of-concept, phage \u03d5Ph_SE03 was produced in ST155 by employing a unique Bacteriophage Amplification Reactor-Lytics Broadcasting System and was simultaneously disseminated into <jats:italic>S. enterica<\/jats:italic> augmented wastewater, which resulted in a 3-log reduction in 24\u00a0h. 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