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Subsequently, the presence of the gene encoding A subunit of cholera toxin (<jats:italic>ctxA<\/jats:italic>) and the cholera toxin (CT) level in these stool samples were examined.<jats:italic>ctxA<\/jats:italic>was examined by both metagenomic sequencing analysis and polymerase chain reaction. In these examinations, two samples did not show positive in any of these tests. The metagenomic analysis showed that the genes for<jats:italic>Streptococcus pneumoniae<\/jats:italic>and<jats:italic>Salmonella enterica<\/jats:italic>were present in the stools of these two patients, respectively. Therefore, these two patients were not considered to have diarrhea due to<jats:italic>V. cholerae<\/jats:italic>infection. From these results, we predicted that some Kolkata residents harbor a small number of<jats:italic>V. cholerae<\/jats:italic>in their intestines as a form of subclinical infection with<jats:italic>V. cholerae<\/jats:italic>. Next, we analyzed the stool samples of 22 diarrhea patients from which<jats:italic>V. cholerae<\/jats:italic>was not isolated. The results showed that 3 of the patients seemed to have subclinical infection of<jats:italic>V. cholerae<\/jats:italic>based on the amount of the genes. These results indicated that subclinical infections with<jats:italic>V. cholerae<\/jats:italic>O1 occur in Kolkata.<\/jats:p>","DOI":"10.1038\/s41598-022-24167-9","type":"journal-article","created":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T14:06:33Z","timestamp":1668434793000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Metagenomic analysis of diarrheal stools in Kolkata, India, indicates the possibility of subclinical infection of Vibrio cholerae O1"],"prefix":"10.1038","volume":"12","author":[{"given":"Eizo","family":"Takahashi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Motooka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shota","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin-ichi","family":"Miyoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goutam","family":"Chowdhury","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asish K.","family":"Mukhopadhyay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanta","family":"Dutta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daichi","family":"Morita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Iida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keinosuke","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,11,14]]},"reference":[{"key":"24167_CR1","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/1757-4749-2-4","volume":"2","author":"GB Nair","year":"2010","unstructured":"Nair, G. 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