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In this paper, we report on the variable region of class 1 and 2 integrons observed in MDR <jats:italic>A. baumanni<\/jats:italic> isolates recovered from rivers in the Eastern Cape Province, South Africa. Class 1 and 2 integrons with their variable regions were evaluated with polymerase chain reaction techniques followed by sequencing. Antibiotic sensitivity testing, checkerboard assay, time-kill independent assay, and Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) were carried out using standard microbiological techniques. A total of fifty-six (56) isolates were examined, among which 45 (79%) tested positive for class 1 integron, and 7 (12.3%) had class 2 integron. None was found to be class 3 integron positive among the isolates. The variable region contained <jats:italic>aadA1, aadA5<\/jats:italic>, and <jats:italic>aadA2<\/jats:italic> genes, which confer resistance against streptomycin and spectinomycin, <jats:italic>aac(6\u2032)-Ib<\/jats:italic> against amikacin\/ tobramycin and <jats:italic>dfrA17<\/jats:italic> genes against trimethoprim. The minimum inhibitory concentrations of the antimicrobials for one of the tested organisms were resistant against meropenem, colistin sodium methanesulfonate, tetracycline, ceftazidime, and ciprofloxacin (16,\u2009&gt;\u200916,\u2009&gt;\u20098,\u2009&gt;\u2009256, and 128 ug\/mL respectively). The impact of colistin combined with quinolones (ciprofloxacin), with the FICIs (0.31) indicated synergistic effects against MDR <jats:italic>A baumanni<\/jats:italic>. However, when colistin was combined with meropenem and ceftazidime, additive effects with fractional inhibitory concentration (FIC) index ranging from 0.52 to 1 were observed. No antagonistic effect was evaluated among the examined isolates. ERIC-PCR analyses of <jats:italic>A. baumanni<\/jats:italic> isolates revealed significant genetic diversity, suggesting various sources of environmental contamination. We conclude that <jats:italic>A. baumanni<\/jats:italic> harbouring class 1 integrons in aquatic milieus are a significant source of ARGs and can transmit these elements to other organisms and consequently to man with significant public health implications.<\/jats:p>","DOI":"10.1038\/s41598-022-24724-2","type":"journal-article","created":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T21:32:02Z","timestamp":1669411922000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Prevalence of classes 1 and 2 integrons in multidrug-resistant Acinetobacter baumanni isolates recovered from some aquatic environment in South Africa"],"prefix":"10.1038","volume":"12","author":[{"given":"Oluwaseun Ola","family":"Adeniji","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsiddig A. 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