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The strain <jats:italic>Pseudomonas veronii<\/jats:italic> 7\u201341 can grow on medium-chain <jats:italic>n<\/jats:italic>-alkanes (C<jats:sub>8<\/jats:sub>\u2013C<jats:sub>12<\/jats:sub>) and polycyclic aromatic hydrocarbons such as naphthalene. We performed a genetic analysis and physiological\/biochemical characterization of strain 7\u201341 cultivated in a mineral medium with decane, naphthalene or a mixture of the hydrocarbons. The genes responsible for the degradation of alkanes and PAHs are on the IncP-7 conjugative plasmid and are organized into the <jats:italic>alk<\/jats:italic> and <jats:italic>nah<\/jats:italic> operons typical of pseudomonads. A natural plasmid carrying functional operons for the degradation of two different classes of hydrocarbons was first described. In monosubstrate systems, 28.4% and 68.8% of decane and naphthalene, respectively, were biodegraded by the\u00a0late stationary growth phase. In a bisubstrate system, these parameters were 25.4% and 20.8% by the end of the exponential growth phase. Then the biodegradation stopped, and the bacterial culture started dying due to the accumulation of salicylate (naphthalene-degradation metabolite), which is toxic in high concentrations. The activity of the salicylate oxidation enzymes was below the detection limit. These results indicate that the presence of decane and a high concentration of salicylate lead to impairment of hydrocarbon degradation by the strain.<\/jats:p>","DOI":"10.1038\/s41598-022-25191-5","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T19:04:02Z","timestamp":1669662242000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Pseudomonas veronii strain 7\u201341 degrading medium-chain n-alkanes and polycyclic aromatic hydrocarbons"],"prefix":"10.1038","volume":"12","author":[{"given":"S. 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