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This gripper is built on Polydimethylsiloxane (PDMS) with the soft material casting technique to form three finger-like columns, which are placed on a circular membrane. The fingers have a length of 1.5\u00a0mm and a diameter of 300\u00a0\u00b5m each; the distance between the two fingers is 600\u00a0\u00b5m of center-to-center distance. A membrane as a 150\u00a0\u00b5m soft film is built on top of a cylindrical hollow space. Applying pressure to the interior space can bend the membrane. Bending the membrane causes the motion of opening\/closing of the gripper, and as a result, the three fingers can grip an object or release it. The PDMS was characterized, and the experimental results were used later in Abaqus software to simulate the gripping motion. The range of deformation of the gripper was investigated by simulation and experiment. The result of the simulation agrees with the experiments. The maximum 543 \u00b5N force was measured for this microfluidic-compatible microgripper and it could lift a ball that weighs 168.4\u00a0mg and has a 0.5\u00a0mm diameter. Using this microgripper, an ant was manipulated successfully without any damage. Results showed fabricated device has great a potential as micro\/bio manipulator.<\/jats:p>","DOI":"10.1038\/s41598-022-25713-1","type":"journal-article","created":{"date-parts":[[2022,12,10]],"date-time":"2022-12-10T13:03:14Z","timestamp":1670677394000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["A hydraulic soft microgripper for biological studies"],"prefix":"10.1038","volume":"12","author":[{"given":"Sina","family":"Baghbani Kordmahale","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Qu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia","family":"Muliana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Kameoka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,12,10]]},"reference":[{"issue":"1","key":"25713_CR1","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1097\/MOU.0b013e32831a478c","volume":"19","author":"AM Okamura","year":"2009","unstructured":"Okamura, A. 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