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Among all isolates, two LAB strains isolated from human mother milk and cottage cheese revealed antimicrobial activity against some tested pathogenic strains. Both isolates inhibited all the tested pathogens except\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . The two isolates were identified by morphological, biochemical properties and then by 16S rRNA gene sequencing technique as\n                    <jats:italic>Lactobacillus acidophilus<\/jats:italic>\n                    SAM1 and\n                    <jats:italic>Lactiplantibacillus plantarum<\/jats:italic>\n                    SAM2. Potential probiotic characters were investigated. Both strains survived in relatively low pH and high bile concentrations and were able to grow at 0.5% of pancreatin concentrations. Their growth decreased by increasing phenol from 0.2% till 0.5%. Both strains did not show hemolytic activity. Coaggregation potential was exhibited by the two strains against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    and\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    . Hydrophobicity of\n                    <jats:italic>Lactobacillus acidophilus<\/jats:italic>\n                    SAM1 and\n                    <jats:italic>Lactiplantibacillus plantarum<\/jats:italic>\n                    SAM2, with ethyl acetate; were 88.1% and 82.8%, respectively.\n                    <jats:italic>Lactobacillus acidophilus<\/jats:italic>\n                    SAM1 was susceptible to Ampicillin, Penicillin, Erythromycin, Ciprofloxacin and Tetracycline; on the contrary, it resists Vancomycin and Cefoxitin; while\n                    <jats:italic>Lactiplantibacillus plantarum<\/jats:italic>\n                    SAM2 resists all tested antibiotics. Maximum growth was achieved using glucose as a carbon source and yeast extract as nitrogen source for both strains; however, glucose is the most preferred carbon source for microorganisms and it prevents the uptake of carbon from other sources like yeast by catabolite repression mechanism.\n                    <jats:italic>Lactobacillus acidophilus<\/jats:italic>\n                    SAM1 produces lipase enzyme, while\n                    <jats:italic>Lactiplantibacillus plantarum<\/jats:italic>\n                    SAM2 produces amylase and protease.\n                  <\/jats:p>","DOI":"10.1038\/s41598-022-27003-2","type":"journal-article","created":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T06:02:48Z","timestamp":1672293768000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Identification of Lactobacillus strains from human mother milk and cottage cheese revealed potential probiotic properties with enzymatic activity"],"prefix":"10.1038","volume":"12","author":[{"given":"Abeer I. 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