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However, it remains unclear if this mechanical interaction is affected by the stiffness of the structural connection. This study therefore aimed to investigate the impact of the knee angle on myofascial force transmission across the dorsal knee. A randomized, cross-over study was performed, including n\u2009=\u200956 healthy participants (25.36\u2009\u00b1\u20093.9\u00a0years, 25 females). On two separate days, they adopted a prone position on an isokinetic dynamometer (knee extended or 60\u00b0 flexed). In each condition, the device moved the ankle three times from maximal plantarflexion to maximal dorsal extension. Muscle inactivity was ensured using EMG. High-resolution ultrasound videos of the semimembranosus (SM) and the gastrocnemius medialis (GM) soft tissue were recorded. Maximal horizontal tissue displacement, obtained using cross-correlation, was examined as a surrogate of force transmission. SM tissue displacement was higher at extended (4.83\u2009\u00b1\u20092.04\u00a0mm) than at flexed knees (3.81\u2009\u00b1\u20092.36\u00a0mm). Linear regression demonstrated significant associations between (1) SM and GM soft tissue displacement (extended: R<jats:sup>2<\/jats:sup>\u2009=\u20090.18, p\u2009=\u20090.001; flexed: R<jats:sup>2<\/jats:sup>\u2009=\u20090.17, p\u2009=\u20090.002) as well as (2) SM soft tissue displacement and ankle range of motion (extended: R<jats:sup>2<\/jats:sup>\u2009=\u20090.103, p\u2009=\u20090.017; flexed: R<jats:sup>2<\/jats:sup>\u2009=\u20090.095, p\u2009=\u20090.022). Our results further strengthen the evidence that local stretching induces a force transmission to neighboring muscles. Resulting remote exercise effects such as increased range of motion, seem to depend on the stiffness of the continuity.<\/jats:p><jats:p>Trial registration: DRKS (Deutsches Register Klinischer Studien), registration number DRKS00024420, first registered 08\/02\/2021, <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/drks.de\/search\/de\/trial\/DRKS00024420\">https:\/\/drks.de\/search\/de\/trial\/DRKS00024420<\/jats:ext-link>.<\/jats:p>","DOI":"10.1038\/s41598-023-30407-3","type":"journal-article","created":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T13:07:27Z","timestamp":1678108047000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Myofascial force transmission between the calf and the dorsal thigh is dependent on knee angle: an ultrasound study"],"prefix":"10.1038","volume":"13","clinical-trial-number":[{"clinical-trial-number":"drks00024420","registry":"10.18810\/drks"}],"author":[{"given":"Lisa","family":"Mohr","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lutz","family":"Vogt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Thiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Behringer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Wilke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,3,6]]},"reference":[{"key":"30407_CR1","doi-asserted-by":"publisher","first-page":"12623","DOI":"10.1038\/s41598-021-92194-z","volume":"11","author":"C Fede","year":"2021","unstructured":"Fede, C. et al. 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