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In the present study, we aimed to screen the functionally active phosphate solubilizing bacteria (PSB) associated with\n                    <jats:italic>Dalbergia sissoo Roxb.<\/jats:italic>\n                    (Shisham) from different provenances. Screening for phosphate solubilization was done on Pikovskaya's agar, and 18 bacteria positive for the tri-calcium phosphate (Ca\n                    <jats:sub>3<\/jats:sub>\n                    (PO\n                    <jats:sub>4<\/jats:sub>\n                    )\n                    <jats:sub>2<\/jats:sub>\n                    solubilization showing visible dissolution halo zones were identified. All 18 isolates showed zinc solubilization, indole acetic acid (IAA), siderophore, and hydrogen cyanide (HCN) production. The morphological and biochemical characterization with 16S\u00a0rDNA gene-based phylogenetic analysis identified bacterial strains as belonging to the genus\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella, Streptomyces, Pantoea, Kitasatospora, Micrococcus,<\/jats:italic>\n                    and\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    . Among all the isolates, one of the isolates named L4, from Lacchiwala region was the most efficient P solubilizer with a high P solubilization index (4.75\u2009\u00b1\u20090.06) and quantitative P solubilization activity (891.38\u2009\u00b1\u200918.55 \u03bcg mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ). The validation of phosphate solubilization activity of PSB isolates was done by amplification of the Pyrroloquinoline quinone (PQQ) genes,\n                    <jats:italic>pqqA<\/jats:italic>\n                    and\n                    <jats:italic>pqqC<\/jats:italic>\n                    . Based on this study, we have selected the bacterial strains which are efficient phosphate solubilizers and could be economical and eco-friendly in plant growth promotion, disease suppression, as an antioxidant, and for subsequent enhancement of yield.\n                  <\/jats:p>","DOI":"10.1038\/s41598-023-33217-9","type":"journal-article","created":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T12:05:16Z","timestamp":1682683516000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Functional characterization and molecular fingerprinting of potential phosphate solubilizing bacterial candidates from Shisham rhizosphere"],"prefix":"10.1038","volume":"13","author":[{"given":"Samiksha","family":"Joshi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saurabh","family":"Gangola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vandana","family":"Jaggi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manvika","family":"Sahgal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,4,28]]},"reference":[{"key":"33217_CR1","doi-asserted-by":"publisher","first-page":"100080","DOI":"10.1016\/j.stress.2022.100080","volume":"4","author":"VK Dhiman","year":"2022","unstructured":"Dhiman, V. 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