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We studied how supplementing\n                    <jats:italic>Labeo rohita<\/jats:italic>\n                    \u2019s diet with reishi mushroom powder containing beta-glucan influenced immunological function. A supplemented diet containing 10% reishi mushroom powder was administered for 120\u00a0days. Afterwards, analyses were conducted on different immunological parameters such as antioxidants, respiratory burst, reactive oxygen species (ROS), alternative complement activity, and serum immunoglobulin, which resulted significant increases (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01) for the reishi mushroom-fed immune primed\n                    <jats:italic>L. rohita<\/jats:italic>\n                    . Additionally, analyzing various hematological parameters such as erythrocytes and leukocytes count were assessed to elucidate the immunomodulatory effects, indicating positive effects of dietary reishi mushroom powder on overall fish health. Furthermore, the bacterial challenge-test with 1.92\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    \u00a0CFU\/ml intramuscular dose of\n                    <jats:italic>Aeromonas veronii<\/jats:italic>\n                    showed enhanced disease-defending system as total serum protein and lysozyme activity levels accelerated significantly (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). Nevertheless, reishi mushroom powder contained with beta-glucan ameliorated the stress indicating parameters like acetylcholinesterase (AChE), serum-glutamic pyruvic transaminase (SGPT) and serum-glutamic oxaloacetic transaminase (SGOT) enzyme activities results suggested the fish\u2019s physiology was unaffected. Therefore, the results indicated that adding dietary reishi mushroom as a source of beta-glucan could significantly boost the immune responses in Rohu.\n                  <\/jats:p>","DOI":"10.1038\/s41598-023-41557-9","type":"journal-article","created":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T12:02:57Z","timestamp":1693915377000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Immuno-physiological effects of dietary reishi mushroom powder as a source of beta-glucan on Rohu, Labeo rohita challenged with Aeromonas veronii"],"prefix":"10.1038","volume":"13","author":[{"given":"Tutul Kumar","family":"Saha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Mariom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanvir","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Moniruzzaman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taesun","family":"Min","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zakir","family":"Hossain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,9,5]]},"reference":[{"key":"41557_CR1","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/s10499-014-9824-0","volume":"23","author":"S Meshram","year":"2015","unstructured":"Meshram, S., Murthy, H. 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