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Recently evolved sex-linked regions offer an opportunity to understand the mechanisms of recombination cessation. This paper analyses such a region on <jats:italic>Silene latifolia<\/jats:italic> (Caryophyllaceae) sex chromosomes, where recombination was suppressed in the last 120 thousand years (\"stratum 3\"). Locating the boundaries of the stratum 3 in <jats:italic>S. latifolia<\/jats:italic> genome sequence revealed that this region is far larger than assumed previously\u2014it is about 14\u00a0Mb long and includes 202 annotated genes. A gradient of X:Y divergence detected in the stratum 3, with divergence increasing proximally, indicates gradual recombination cessation, possibly caused by expansion of pericentromeric recombination suppression (PRS) into the pseudoautosomal region. Expansion of PRS was also the likely cause for the formation of the older stratum 2 on <jats:italic>S. latifolia<\/jats:italic> sex chromosomes. The role of PRS in sex chromosome evolution has been underappreciated, but it may be a significant factor, especially in the species with large chromosomes where PRS is often extensive.<\/jats:p>","DOI":"10.1038\/s41598-024-51153-0","type":"journal-article","created":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T11:04:09Z","timestamp":1705403049000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Evolution of a plant sex chromosome driven by expanding pericentromeric recombination suppression"],"prefix":"10.1038","volume":"14","author":[{"given":"Dmitry A.","family":"Filatov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,1,16]]},"reference":[{"key":"51153_CR1","doi-asserted-by":"publisher","first-page":"1632","DOI":"10.1038\/s41559-019-1050-8","volume":"3","author":"B Vicoso","year":"2019","unstructured":"Vicoso, B. 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