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Herein, chia gum (CG)\/alginate (A)-polymeric complex was synthesized and employed as a support material for the immobilization of horseradish peroxidase (HRP). HRP was successfully immobilized on the developed ACG-polymeric support, and the highest immobilization recovery (75%) was observed at 1.0% CG and 2% A, pH 7.0, and 50 units of the enzyme. The structure, morphology, and thermal properties of the prepared ACG-HRP were demonstrated using Fourier Transform Infrared (FTIR), Scanning Electron Microscope, and Thermogravimetric (TGA) analyses. ACG-HRP showed a good reusability (60%) over ten reuses. The immobilized ACG-HRP displayed an acidic pH optimum (6.0), a higher temperature optimum (50\u00a0\u00b0C), and improved thermal stability (30\u201350\u00a0\u00b0C) compared to the soluble HRP at pH 7.0, 40\u00a0\u00b0C and (30\u201340\u00a0\u00b0C), respectively. ACG-HRP has a lower affinity for hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) and guaiacol and a higher oxidizing affinity for a number of phenolic substrates. The ACG-HRP demonstrated greater resistance to heavy metals, isopropanol, urea, Triton X-100, and urea, as well as improved efficiency for eliminating phenol and <jats:italic>p<\/jats:italic>-chlorophenol. The developed ACG-polymeric support provided improved enzyme properties, allowed the reuse of the immobilized HRP in 10 cycles, and made it promising for several biotechnological applications.<\/jats:p>","DOI":"10.1038\/s41598-024-51566-x","type":"journal-article","created":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T10:03:36Z","timestamp":1705399416000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Development of chia gum\/alginate-polymer support for horseradish peroxidase immobilization and its application in phenolic removal"],"prefix":"10.1038","volume":"14","author":[{"given":"Saleh A.","family":"Mohamed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alshaimaa M.","family":"Elsayed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hala A.","family":"Salah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal Z.","family":"Barakat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roqaya I.","family":"Bassuiny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi M.","family":"Abdel-Mageed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azza M.","family":"Abdel-Aty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,1,16]]},"reference":[{"key":"51566_CR1","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.jgeb.2013.07.001","volume":"11","author":"AM Abdel-Aty","year":"2013","unstructured":"Abdel-Aty, A. 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