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Long noncoding RNAs (lncRNAs) have emerged as pivotal regulators of gene expression, potentially shedding light on NSCL\/P's etiology. This study aimed to identify critical lncRNAs and construct regulatory networks to unveil NSCL\/P's underlying molecular mechanisms. Integrating gene expression profiles from the Gene Expression Omnibus (GEO) database, we pinpointed 30 dysregulated NSCL\/P-associated lncRNAs. Subsequent analyses enabled the creation of competing endogenous RNA (ceRNA) networks, lncRNA-RNA binding protein (RBP) interaction networks, and lncRNA cis and trans regulation networks. RT-qPCR was used to examine the regulatory networks of lncRNA in vivo and in vitro. Furthermore, protein levels of lncRNA target genes were validated in human NSCL\/P tissue samples and murine palatal shelves. Consequently, two lncRNAs and three mRNAs: <jats:italic>FENDRR<\/jats:italic> (log2FC\u2009=\u2009\u2212\u00a00.671, P\u2009=\u20090.040), <jats:italic>TPT1-AS1<\/jats:italic> (log2FC\u2009=\u20090.854, P\u2009=\u20090.003), <jats:italic>EIF3H<\/jats:italic> (log2FC\u2009=\u2009\u2212\u00a01.081, P\u2009=\u20090.041), <jats:italic>RBBP6<\/jats:italic> (log2FC\u2009=\u20090.914, P\u2009=\u20090.037), and <jats:italic>SRSF1<\/jats:italic> (log2FC\u2009=\u20090.763, P\u2009=\u20090.026) emerged as potential contributors to NSCL\/P pathogenesis. Functional enrichment analyses illuminated the biological functions and pathways associated with these lncRNA-related networks in NSCL\/P. In summary, this study comprehensively delineates the dysregulated transcriptional landscape, identifies associated lncRNAs, and reveals pivotal sub-networks relevant to NSCL\/P development, aiding our understanding of its molecular progression and setting the stage for further exploration of lncRNA and mRNA regulation in NSCL\/P.<\/jats:p>","DOI":"10.1038\/s41598-024-51747-8","type":"journal-article","created":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T19:02:03Z","timestamp":1704913323000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Unveiling dysregulated lncRNAs and networks in non-syndromic cleft lip with or without cleft palate pathogenesis"],"prefix":"10.1038","volume":"14","author":[{"given":"Caihong","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojie","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuorong","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengnan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawen","family":"You","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqing","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,1,10]]},"reference":[{"key":"51747_CR1","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1038\/nrg2933","volume":"12","author":"MJ Dixon","year":"2011","unstructured":"Dixon, M. 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