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Fortunately, Brazil has good accessibility to high-quality antivenoms at the genus and inter-genus level, enabling the treatment of many\u00a0of these envenomings. However, the optimal use of these treatments requires that the snake species responsible for the bite is determined. Currently,\u00a0physicians use a syndromic approach to diagnose snakebite, which can be difficult for medical personnel with limited training in clinical snakebite management. In this work, we have developed a novel monoclonal antibody-based multiplex lateral flow assay for differentiating <jats:italic>Bothrops<\/jats:italic> and <jats:italic>Lachesis<\/jats:italic> venoms within 15\u00a0min. The test can be read by the naked eye or (semi)-quantitatively by a smartphone supported by a 3D-printed attachment for controlling lighting conditions. The LFA can detect <jats:italic>Bothrops<\/jats:italic> and <jats:italic>Lachesis<\/jats:italic> venoms in spiked plasma and urine matrices at concentrations spanning six orders of magnitude. The LFA has detection limits of 10\u201350\u00a0ng\/mL in spiked plasma and urine, and 50\u2013500\u00a0ng\/mL in spiked sera, for <jats:italic>B. atrox<\/jats:italic> and <jats:italic>L. muta<\/jats:italic> venoms. This test could potentially support medical personnel in correctly diagnosing snakebite envenomings at the point-of-care in Brazil, which may help improve patient outcomes and save lives.<\/jats:p>","DOI":"10.1038\/s41598-024-51971-2","type":"journal-article","created":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T09:04:23Z","timestamp":1706691863000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Multiplex lateral flow assay development for snake venom detection in biological matrices"],"prefix":"10.1038","volume":"14","author":[{"given":"Cecilie","family":"Knudsen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selma B.","family":"Belfakir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pelle","family":"Degnegaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas A.","family":"J\u00fcrgensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksander M.","family":"Haack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasmus U. 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Selma Belfakir is employed by VenomAid Diagnostics ApS. Pelle Degnegaard is employed by VenomAid Diagnostics ApS. Jonas A. J\u00fcrgensen is a co-founder\u00a0of, shareholder in\u00a0and employed by VenomAid Diagnostics ApS. S\u00f8ren H. Dam is a co-founder\u00a0of, shareholder\u00a0in and investor in VenomAid Diagnostics ApS. Aleksander M. Haack is a co-founder\u00a0of and shareholder in VenomAid Diagnostics ApS. Rasmus U. W. Friss is a co-founder\u00a0of, shareholder\u00a0in and investor of VenomAid Diagnostics ApS. Prof. Andreas H. Laustsen is a co-founder\u00a0of, shareholder\u00a0in and investor in VenomAid Diagnostics ApS. Dr. Georgina M. S. Ross is employed by VenomAid Diagnostics ApS. Authors declare no other competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"2567"}}