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In Ecuador we compared two different PPD brands for bTB diagnosis using skin testing and measuring\u00a0skin thickness increase. Additionally, we evaluated four PPD brands, including\u00a0those used for skin testing, in the Bovine Tuberculosis Interferon Gamma Test (IFN-\u03b3 test) measuring IFN-\u03b3 induction in whole blood. The study included 17 naturally tuberculosis-infected PPD and IFN-\u03b3 test positive bovines. Both the field and laboratory results showed significant differences in classifying the 17 bovines as bTB positive or negative. We hypothesize that several factors, such as the genetic background of the cows, sensitization to\u00a0environmental mycobacteria, <jats:italic>M. bovis<\/jats:italic> strains involved in the bTB infection, and the\u00a0manufacturing procedures of the PPDs, could have influenced the immune reaction toward the different tuberculin PPD brands. 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