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The alkaloid piperine, is the key phytoconstituent of the plant, primarily responsible for its\u2019 pharmacological-impacts. The aim of the study is to analyse the intra-specific variation in piperine content among different chemotypes (PL1 to PL 30) and identify high piperine yielding chemotype (elite-chemotype) collected from 10 different geographical regions of West Bengal by validated HPTLC chromatography method. The study also focused on the pharmacological-screening to better understand the antioxidant activity of the methanol extracts of\n                    <jats:italic>P. longum<\/jats:italic>\n                    by DPPH and ABTS radical-scavenging activity and genotoxic activity by\n                    <jats:italic>Allium cepa<\/jats:italic>\n                    root tip assay. It was found that the\n                    <jats:italic>P. longum<\/jats:italic>\n                    fruit chemotypes contain high amount piperine (highest 16.362\u00a0mg\/g in chemotype PL9) than the stem and leaf chemotypes. Both DPPH and ABTS antioxidant assays revealed that\n                    <jats:italic>P. longum<\/jats:italic>\n                    showed moderate radical-scavenging activity and the highest activity was found in PL9 (fruit) chemotype with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 124.2\n                    <jats:inline-formula>\n                      <jats:alternatives>\n                        <jats:tex-math>$$\\pm 0.97$$<\/jats:tex-math>\n                        <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\">\n                          <mml:mrow>\n                            <mml:mo>\u00b1<\/mml:mo>\n                            <mml:mn>0.97<\/mml:mn>\n                          <\/mml:mrow>\n                        <\/mml:math>\n                      <\/jats:alternatives>\n                    <\/jats:inline-formula>\n                    and 104\n                    <jats:inline-formula>\n                      <jats:alternatives>\n                        <jats:tex-math>$$\\pm 0.78$$<\/jats:tex-math>\n                        <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\">\n                          <mml:mrow>\n                            <mml:mo>\u00b1<\/mml:mo>\n                            <mml:mn>0.78<\/mml:mn>\n                          <\/mml:mrow>\n                        <\/mml:math>\n                      <\/jats:alternatives>\n                    <\/jats:inline-formula>\n                    \u00b5g\/ml respectively. The\n                    <jats:italic>A. cepa<\/jats:italic>\n                    root tip assay showed no such significant genotoxic-effect and change in mitotic-index. The quick, reproducible, and validated HPTLC approach offers a useful tool for determining quantitative variations of piperine among\n                    <jats:italic>P. longum<\/jats:italic>\n                    chemotypes from different geographical-regions and also according to the different tissues and choose elite genotypes with high piperine production for continued propagation and commercialization for the pharmaceutical sector. Additionally, the plant's\n                    <jats:italic>in-vitro<\/jats:italic>\n                    antioxidant property and lack of genotoxicity directly supports its\u2019 widespread and long history of use as a medicinal and culinary plant.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-52297-9","type":"journal-article","created":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:05:06Z","timestamp":1709251506000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Tissue-specific variations of piperine in ten populations of Piper longum L.: bioactivities and toxicological profile"],"prefix":"10.1038","volume":"14","author":[{"given":"Protha","family":"Biswas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devendra Kumar","family":"Pandey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahipal S.","family":"Shekhawat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhijit","family":"Dey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tabarak","family":"Malik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,1]]},"reference":[{"issue":"12","key":"52297_CR1","doi-asserted-by":"publisher","first-page":"4425","DOI":"10.1002\/ptr.7649","volume":"36","author":"P Biswas","year":"2022","unstructured":"Biswas, P. et al. 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