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This was a retrospective observational study. One hundred and\u00a0eighty nine eyes of 189 subjects without ocular diseases were examined by enhanced depth imaging (EDI)-OCT. A choroidal OCT horizontal image with a width of 1500\u00a0\u03bcm centered on the fovea was binarized. The lumen, stroma, and total choroidal area in the choriocapillaris (CC), Sattler\u2019s layer (SL), and Haller's layer (HL) were measured, and the ratio of the luminal area to total choroidal area (L\/C ratio) was calculated. Multiple regression analysis was performed for choroidal parameters in each choroidal layer and for age, refractive power, and ocular axial length. Multiple regression analysis showed that an older age was significantly correlated with a lower choroidal area and the L\/C ratio in all choroidal layers (each <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). A Long axial length was significantly associated with lower SL and HL (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), but not with refractive power. In the choroid of normal eyes, age-related decreases in the choroidal area and L\/C ratio were associated with all choroidal layers, and elongation of the axial length was associated with thinning of SL and HL.<\/jats:p>","DOI":"10.1038\/s41598-024-52627-x","type":"journal-article","created":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T08:55:57Z","timestamp":1706691357000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of age and axial length on choroidal stratified structure in normal eyes"],"prefix":"10.1038","volume":"14","author":[{"given":"Yuki","family":"Ito","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Endo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Kase","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuo","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shozo","family":"Sonoda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomonori","family":"Sakoguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiji","family":"Sakamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Ishida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Kase","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,1,30]]},"reference":[{"key":"52627_CR1","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/j.preteyeres.2009.12.002","volume":"29","author":"DL Nickla","year":"2010","unstructured":"Nickla, D. 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