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The vulnerable status severely limits the medicinal value and wide application of dragon\u2019s blood. Therefore, it\u2019s essential to analyze the mechanisms that form dragon\u2019s blood in order to increase artificial production. To clarify the mechanisms forming dragon\u2019s blood, understanding gene expression in the flavonoid biosynthesis pathway is the foundation. However, reference genes of\n                    <jats:italic>D. cochinchinensis<\/jats:italic>\n                    haven\u2019t been analyzed. In this study, expression profiles of seven commonly used housekeeping genes (\n                    <jats:italic>Actin<\/jats:italic>\n                    ,\n                    <jats:italic>\u03b1-EF<\/jats:italic>\n                    ,\n                    <jats:italic>UBC<\/jats:italic>\n                    ,\n                    <jats:italic>\u03b2-tubulin<\/jats:italic>\n                    ,\n                    <jats:italic>18S<\/jats:italic>\n                    ,\n                    <jats:italic>GAPDH<\/jats:italic>\n                    ,\n                    <jats:italic>His<\/jats:italic>\n                    ) were evaluated by using quantitative real-time PCR combined with the algorithms geNorm, NormFinder, BestKeeper, and RefFinder. On the basis of overall stability ranking, the best reference genes were the combinations\n                    <jats:italic>\u03b2-tubulin<\/jats:italic>\n                    \u2009+\n                    <jats:italic>UBC<\/jats:italic>\n                    for wounded stems and\n                    <jats:italic>\u03b1-EF<\/jats:italic>\n                    \u2009+\n                    <jats:italic>18S<\/jats:italic>\n                    \u2009+\u2009\n                    <jats:italic>Actin<\/jats:italic>\n                    for different organs. Reliability of the recommended reference genes was validated by normalizing relative expression of two key enzyme genes\n                    <jats:italic>PAL1<\/jats:italic>\n                    and\n                    <jats:italic>CHI1<\/jats:italic>\n                    in the flavonoid biosynthesis pathway. The results provide a foundation to study gene expression in future research on\n                    <jats:italic>D. cochinchinensis<\/jats:italic>\n                    or other\n                    <jats:italic>Dracaena.<\/jats:italic>\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-52754-5","type":"journal-article","created":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T05:05:52Z","timestamp":1710392752000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Screening and validation of reference genes in Dracaena cochinchinensis using quantitative real-time PCR"],"prefix":"10.1038","volume":"14","author":[{"given":"Shixi","family":"Gao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junxiang","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Rong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhe","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,14]]},"reference":[{"key":"52754_CR1","doi-asserted-by":"publisher","first-page":"508","DOI":"10.1016\/j.jep.2014.01.032","volume":"152","author":"N Li","year":"2014","unstructured":"Li, N., Ma, Z., Li, M., Xing, Y. & Hou, Y. 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