{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:04:53Z","timestamp":1772136293779,"version":"3.50.1"},"reference-count":87,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T00:00:00Z","timestamp":1706486400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T00:00:00Z","timestamp":1706486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000090","name":"Congressionally Directed Medical Research Programs","doi-asserted-by":"publisher","award":["W81XWH-16-1-0580"],"award-info":[{"award-number":["W81XWH-16-1-0580"]}],"id":[{"id":"10.13039\/100000090","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R01HL141761-01"],"award-info":[{"award-number":["1R01HL141761-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Therapies to revascularize ischemic tissue have long been a goal for the treatment of vascular disease and other disorders. Therapies using stem cell factor (SCF), also known as a c-Kit ligand, had great promise for treating ischemia for myocardial infarct and stroke, however clinical development for SCF was stopped due to toxic side effects including mast cell activation in patients. We recently developed a novel therapy using a transmembrane form of SCF (tmSCF) delivered in lipid nanodiscs. In previous studies, we demonstrated tmSCF nanodiscs were able to induce revascularization of ischemia limbs in mice and did not activate mast cells. To advance this therapeutic towards clinical application, we tested this therapy in an advanced model of hindlimb ischemia in rabbits with hyperlipidemia and diabetes. This model has therapeutic resistance to angiogenic therapies and maintains long term deficits in recovery from ischemic injury. We treated rabbits with local treatment with tmSCF nanodiscs or control solution delivered locally from an alginate gel delivered into the ischemic limb of the rabbits. After eight weeks, we found significantly higher vascularity in the tmSCF nanodisc-treated group in comparison to alginate treated control as quantified through angiography. Histological analysis also showed a significantly higher number of small and large blood vessels in the ischemic muscles of the tmSCF nanodisc treated group. Importantly, we did not observe inflammation or mast cell activation in the rabbits. Overall, this study supports the therapeutic potential of tmSCF nanodiscs for treating peripheral ischemia.<\/jats:p>","DOI":"10.1038\/s41598-024-52888-6","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T01:02:10Z","timestamp":1706490130000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Transmembrane stem factor nanodiscs enhanced revascularization in a hind limb ischemia model in diabetic, hyperlipidemic rabbits"],"prefix":"10.1038","volume":"14","author":[{"given":"Eri","family":"Takematsu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miles","family":"Massidda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gretchen","family":"Howe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Goldman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Felli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Mei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory","family":"Callahan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew D.","family":"Sligar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Smalling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron B.","family":"Baker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,1,29]]},"reference":[{"key":"52888_CR1","doi-asserted-by":"publisher","first-page":"435","DOI":"10.7150\/ijbs.6087","volume":"9","author":"J Liang","year":"2013","unstructured":"Liang, J. et al. The C-kit receptor-mediated signal transduction and tumor-related diseases. Int. J. Biol. Sci. 9, 435\u2013443. https:\/\/doi.org\/10.7150\/ijbs.6087 (2013).","journal-title":"Int. J. Biol. Sci."},{"key":"52888_CR2","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.molmed.2013.12.004","volume":"20","author":"K Futrega","year":"2014","unstructured":"Futrega, K., King, M., Lott, W. B. & Doran, M. R. Treating the whole not the hole: necessary coupling of technologies for diabetic foot ulcer treatment. Trends Mol. Med. 20, 137\u2013142. https:\/\/doi.org\/10.1016\/j.molmed.2013.12.004 (2014).","journal-title":"Trends Mol. Med."},{"key":"52888_CR3","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.7150\/ijbs.7502","volume":"9","author":"P Rajendran","year":"2013","unstructured":"Rajendran, P. et al. The vascular endothelium and human diseases. Int. J. Biol. Sci. 9, 1057\u20131069. https:\/\/doi.org\/10.7150\/ijbs.7502 (2013).","journal-title":"Int. J. Biol. Sci."},{"key":"52888_CR4","doi-asserted-by":"publisher","first-page":"S285","DOI":"10.2337\/dc11-s239%JDiabetesCare","volume":"34","author":"A Avogaro","year":"2011","unstructured":"Avogaro, A., Albiero, M., Menegazzo, L., de Kreutzenberg, S. & Fadini, G. P. Endothelial dysfunction in diabetes. Role Reparat. Mech. 34, S285\u2013S290. https:\/\/doi.org\/10.2337\/dc11-s239%JDiabetesCare (2011).","journal-title":"Role Reparat. Mech."},{"key":"52888_CR5","doi-asserted-by":"publisher","first-page":"961","DOI":"10.4239\/wjd.v6.i7.961","volume":"6","author":"T Thiruvoipati","year":"2015","unstructured":"Thiruvoipati, T., Kielhorn, C. E. & Armstrong, E. J. Peripheral artery disease in patients with diabetes: Epidemiology, mechanisms, and outcomes. World J. Diabetes 6, 961\u2013969. https:\/\/doi.org\/10.4239\/wjd.v6.i7.961 (2015).","journal-title":"World J. Diabetes"},{"key":"52888_CR6","first-page":"12","volume":"30","author":"FJ Tursi","year":"2017","unstructured":"Tursi, F. J. & Walker, C. Essential keys to diagnosing and treating PAD in patients with diabetes. Podiatry Today 30, 12\u201317 (2017).","journal-title":"Podiatry Today"},{"key":"52888_CR7","first-page":"41","volume":"19","author":"CR Na","year":"2011","unstructured":"Na, C. R., Wang, S., Kirsner, R. & Federman, G. The elderly and peripheral arterial disease. Clin. Geriatrics. 19, 41\u201346 (2011).","journal-title":"Clin. Geriatrics."},{"key":"52888_CR8","doi-asserted-by":"publisher","first-page":"863","DOI":"10.1038\/nrd1226","volume":"2","author":"M Simons","year":"2003","unstructured":"Simons, M. & Ware, J. A. Therapeutic angiogenesis in cardiovascular disease. Nat. Rev. Drug Discov. 2, 863\u2013871. https:\/\/doi.org\/10.1038\/nrd1226 (2003).","journal-title":"Nat. Rev. Drug Discov."},{"key":"52888_CR9","doi-asserted-by":"publisher","first-page":"1627","DOI":"10.1161\/ATVBAHA.120.312862","volume":"40","author":"JP Cooke","year":"2020","unstructured":"Cooke, J. P. & Meng, S. Vascular regeneration in peripheral artery disease. Arterioscler. Thromb. Vasc. Biol. 40, 1627\u20131634. https:\/\/doi.org\/10.1161\/ATVBAHA.120.312862 (2020).","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"52888_CR10","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1038\/nrcardio.2013.70","volume":"10","author":"BH Annex","year":"2013","unstructured":"Annex, B. H. Therapeutic angiogenesis for critical limb ischaemia. Nat. Rev. Cardiol. 10, 387\u2013396. https:\/\/doi.org\/10.1038\/nrcardio.2013.70 (2013).","journal-title":"Nat. Rev. Cardiol."},{"key":"52888_CR11","doi-asserted-by":"publisher","first-page":"1345","DOI":"10.1182\/blood.V90.4.1345","volume":"90","author":"VC Broudy","year":"1997","unstructured":"Broudy, V. C. Stem cell factor and hematopoiesis. Blood 90, 1345\u20131364. https:\/\/doi.org\/10.1182\/blood.V90.4.1345 (1997).","journal-title":"Blood"},{"key":"52888_CR12","doi-asserted-by":"publisher","first-page":"1619","DOI":"10.1152\/physrev.00046.2011","volume":"92","author":"J Lennartsson","year":"2012","unstructured":"Lennartsson, J. & Ronnstrand, L. Stem cell factor receptor\/c-Kit: from basic science to clinical implications. Physiol. Rev. 92, 1619\u20131649. https:\/\/doi.org\/10.1152\/physrev.00046.2011 (2012).","journal-title":"Physiol. Rev."},{"key":"52888_CR13","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1038\/nature10783","volume":"481","author":"L Ding","year":"2012","unstructured":"Ding, L., Saunders, T. L., Enikolopov, G. & Morrison, S. J. Endothelial and perivascular cells maintain haematopoietic stem cells. Nature 481, 457\u2013462. https:\/\/doi.org\/10.1038\/nature10783 (2012).","journal-title":"Nature"},{"key":"52888_CR14","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1182\/blood.V84.2.408.408","volume":"84","author":"CL Li","year":"1994","unstructured":"Li, C. L. & Johnson, G. R. Stem cell factor enhances the survival but not the self-renewal of murine hematopoietic long-term repopulating cells. Blood 84, 408\u2013414 (1994).","journal-title":"Blood"},{"key":"52888_CR15","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1002\/stem.160112","volume":"16","author":"RV Gardner","year":"1998","unstructured":"Gardner, R. V., Oliver, P. & Astle, C. M. Stem cell factor improves the repopulating ability of primitive hematopoietic stem cells after sublethal irradiation (and, to a lesser extent) after bone marrow transplantation in mice. Stem Cells 16, 112\u2013119. https:\/\/doi.org\/10.1002\/stem.160112 (1998).","journal-title":"Stem Cells"},{"key":"52888_CR16","doi-asserted-by":"publisher","first-page":"9464","DOI":"10.1073\/pnas.89.20.9464","volume":"89","author":"KM Zsebo","year":"1992","unstructured":"Zsebo, K. M. et al. Radioprotection of mice by recombinant rat stem cell factor. Proc. Natl. Acad. Sci. U. S. A. 89, 9464\u20139468. https:\/\/doi.org\/10.1073\/pnas.89.20.9464 (1992).","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"52888_CR17","doi-asserted-by":"publisher","first-page":"2","DOI":"10.2174\/1574889811308010002","volume":"8","author":"LR Zhao","year":"2013","unstructured":"Zhao, L. R., Piao, C. S., Murikinati, S. R. & Gonzalez-Toledo, M. E. The role of stem cell factor and granulocyte-colony stimulating factor in treatment of stroke. Recent Pat. CNS Drug Discov. 8, 2\u201312. https:\/\/doi.org\/10.2174\/1574889811308010002 (2013).","journal-title":"Recent Pat. CNS Drug Discov."},{"key":"52888_CR18","doi-asserted-by":"publisher","first-page":"104561","DOI":"10.1016\/j.nbd.2019.104561","volume":"132","author":"S Ping","year":"2019","unstructured":"Ping, S. et al. Stem cell factor and granulocyte colony-stimulating factor promote brain repair and improve cognitive function through VEGF-A in a mouse model of CADASIL. Neurobiol. of Dis. 132, 104561. https:\/\/doi.org\/10.1016\/j.nbd.2019.104561 (2019).","journal-title":"Neurobiol. of Dis."},{"key":"52888_CR19","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD005207.pub4","author":"PMW Bath","year":"2013","unstructured":"Bath, P. M. W., Sprigg, N. & England, T. Colony stimulating factors (including erythropoietin, granulocyte colony stimulating factor and analogues) for stroke. Cochrane Database Syst. Rev. https:\/\/doi.org\/10.1002\/14651858.CD005207.pub4 (2013).","journal-title":"Cochrane Database Syst. Rev."},{"key":"52888_CR20","doi-asserted-by":"publisher","first-page":"1434","DOI":"10.1161\/CIRCRESAHA.111.263830","volume":"111","author":"E Yaniz-Galende","year":"2012","unstructured":"Yaniz-Galende, E. et al. Stem cell factor gene transfer promotes cardiac repair after myocardial infarction via in situ recruitment and expansion of c-kit+ cells. Circ. Res. 111, 1434\u20131445. https:\/\/doi.org\/10.1161\/CIRCRESAHA.111.263830 (2012).","journal-title":"Circ. Res."},{"key":"52888_CR21","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1172\/JCI116164","volume":"91","author":"SJ Galli","year":"1993","unstructured":"Galli, S. J. et al. Reversible expansion of primate mast cell populations in vivo by stem cell factor. J. Clin. Invest. 91, 148\u2013152. https:\/\/doi.org\/10.1172\/JCI116164 (1993).","journal-title":"J. Clin. Invest."},{"key":"52888_CR22","first-page":"427","volume":"86","author":"AM Taylor","year":"1995","unstructured":"Taylor, A. M., Galli, S. J. & Coleman, J. W. Stem-cell factor, the kit ligand, induces direct degranulation of rat peritoneal mast cells in vitro and in vivo: Dependence of the in vitro effect on period of culture and comparisons of stem-cell factor with other mast cell-activating agents. Immunology 86, 427\u2013433 (1995).","journal-title":"Immunology"},{"key":"52888_CR23","doi-asserted-by":"publisher","first-page":"2681","DOI":"10.1084\/jem.183.6.2681","volume":"183","author":"JJ Costa","year":"1996","unstructured":"Costa, J. J. et al. Recombinant human stem cell factor (kit ligand) promotes human mast cell and melanocyte hyperplasia and functional activation in vivo. J. Exp. Med. 183, 2681\u20132686. https:\/\/doi.org\/10.1084\/jem.183.6.2681 (1996).","journal-title":"J. Exp. Med."},{"key":"52888_CR24","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1111\/j.1472-8206.2005.00390.x","volume":"20","author":"CA Da Silva","year":"2006","unstructured":"Da Silva, C. A., Reber, L. & Frossard, N. Stem cell factor expression, mast cells and inflammation in asthma. Fundam. Clin. Pharmacol. 20, 21\u201339. https:\/\/doi.org\/10.1111\/j.1472-8206.2005.00390.x (2006).","journal-title":"Fundam. Clin. Pharmacol."},{"key":"52888_CR25","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1084\/jem.175.1.245","volume":"175","author":"BK Wershil","year":"1992","unstructured":"Wershil, B. K., Tsai, M., Geissler, E. N., Zsebo, K. M. & Galli, S. J. The rat c-kit ligand, stem cell factor, induces c-kit receptor-dependent mouse mast cell activation in vivo. Evidence that signaling through the c-kit receptor can induce expression of cellular function. J. Exp. Med. 175, 245\u2013255. https:\/\/doi.org\/10.1084\/jem.175.1.245 (1992).","journal-title":"J. Exp. Med."},{"key":"52888_CR26","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1161\/01.cir.98.7.687","volume":"98","author":"NG Frangogiannis","year":"1998","unstructured":"Frangogiannis, N. G. et al. Stem cell factor induction is associated with mast cell accumulation after canine myocardial ischemia and reperfusion. Circulation 98, 687\u2013698. https:\/\/doi.org\/10.1161\/01.cir.98.7.687 (1998).","journal-title":"Circulation"},{"key":"52888_CR27","doi-asserted-by":"publisher","first-page":"2497","DOI":"10.1038\/s41467-022-30103-2","volume":"13","author":"E Takematsu","year":"2022","unstructured":"Takematsu, E. et al. Transmembrane stem cell factor protein therapeutics enhance revascularization in ischemia without mast cell activation. Nat. Commun. 13, 2497. https:\/\/doi.org\/10.1038\/s41467-022-30103-2 (2022).","journal-title":"Nat. Commun."},{"key":"52888_CR28","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1016\/j.actbio.2023.06.006","volume":"167","author":"AD Sligar","year":"2023","unstructured":"Sligar, A. D. et al. Syndecan-4 proteoliposomes enhance revascularization in a rabbit hind limb ischemia model of peripheral ischemia. Acta Biomater. 167, 425\u2013435. https:\/\/doi.org\/10.1016\/j.actbio.2023.06.006 (2023).","journal-title":"Acta Biomater."},{"key":"52888_CR29","doi-asserted-by":"publisher","DOI":"10.3791\/58964","author":"AD Sligar","year":"2019","unstructured":"Sligar, A. D. et al. Preclinical model of hind limb ischemia in diabetic rabbits. J .Vis. Exp. https:\/\/doi.org\/10.3791\/58964 (2019).","journal-title":"J .Vis. Exp."},{"key":"52888_CR30","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1182\/blood.V92.3.1055","volume":"92","author":"GT Furuta","year":"1998","unstructured":"Furuta, G. T., Ackerman, S. J., Lu, L., Williams, R. E. & Wershil, B. K. Stem cell factor influences mast cell mediator release in response to eosinophil-derived granule major basic protein. Blood 92, 1055\u20131061 (1998).","journal-title":"Blood"},{"key":"52888_CR31","doi-asserted-by":"publisher","first-page":"2262","DOI":"10.1002\/eji.200323978","volume":"33","author":"AM Feldweg","year":"2003","unstructured":"Feldweg, A. M. et al. gp49B1 suppresses stem cell factor-induced mast cell activation-secretion and attendant inflammation in vivo. Eur. J. Immunol. 33, 2262\u20132268. https:\/\/doi.org\/10.1002\/eji.200323978 (2003).","journal-title":"Eur. J. Immunol."},{"key":"52888_CR32","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1189\/jlb.0713401","volume":"95","author":"AJ MacNeil","year":"2014","unstructured":"MacNeil, A. J., Junkins, R. D., Wu, Z. & Lin, T. J. Stem cell factor induces AP-1-dependent mast cell IL-6 production via MAPK kinase 3 activity. J. Leukoc. Biol. 95, 903\u2013915. https:\/\/doi.org\/10.1189\/jlb.0713401 (2014).","journal-title":"J. Leukoc. Biol."},{"key":"52888_CR33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.intimp.2017.04.017","volume":"48","author":"SY Nam","year":"2017","unstructured":"Nam, S. Y., Kim, H. Y., Kim, H. M. & Jeong, H. J. Betaeta-eudesmol reduces stem cell factor-induced mast cell migration. Int. Immunopharmacol. 48, 1\u20137. https:\/\/doi.org\/10.1016\/j.intimp.2017.04.017 (2017).","journal-title":"Int. Immunopharmacol."},{"key":"52888_CR34","first-page":"2491","volume":"93","author":"EJ Shpall","year":"1999","unstructured":"Shpall, E. J. et al. A randomized phase 3 study of peripheral blood progenitor cell mobilization with stem cell factor and filgrastim in high-risk breast cancer patients. Blood 93, 2491\u20132501 (1999).","journal-title":"Blood"},{"key":"52888_CR35","doi-asserted-by":"publisher","first-page":"1218","DOI":"10.1182\/blood.V94.4.1218","volume":"94","author":"T Facon","year":"1999","unstructured":"Facon, T. et al. Stem cell factor in combination with filgrastim after chemotherapy improves peripheral blood progenitor cell yield and reduces apheresis requirements in multiple myeloma patients: a randomized, controlled trial. Blood 94, 1218\u20131225 (1999).","journal-title":"Blood"},{"key":"52888_CR36","first-page":"253","volume":"35","author":"P Maslak","year":"1998","unstructured":"Maslak, P. & Nimer, S. D. The efficacy of IL-3, SCF, IL-6, and IL-11 in treating thrombocytopenia. Semin. Hematol. 35, 253\u2013260 (1998).","journal-title":"Semin. Hematol."},{"key":"52888_CR37","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.3242","author":"R Fitridge","year":"2019","unstructured":"Fitridge, R., Pena, G. & Mills, J. L. The patient presenting with chronic limb-threatening ischaemia. Does diabetes influence presentation, limb outcomes and survival?. Diabetes Metab. Res. Rev. https:\/\/doi.org\/10.1002\/dmrr.3242 (2019).","journal-title":"Diabetes Metab. Res. Rev."},{"key":"52888_CR38","doi-asserted-by":"publisher","unstructured":"Takahara, M., Iida, O., Kohsaka, S., Soga, Y., Fujihara, M., Shinke, T., Amano, T., Ikari, Y., & JEVT, investigators JP. Diabetes mellitus and other cardiovascular risk factors in lower-extremity peripheral artery disease versus coronary artery disease: an analysis of 1,121,359 cases from the nationwide databases. Cardiovasc. Diabetol. 18, 155. https:\/\/doi.org\/10.1186\/s12933-019-0955-5 (2019).","DOI":"10.1186\/s12933-019-0955-5"},{"key":"52888_CR39","doi-asserted-by":"publisher","first-page":"452095","DOI":"10.1155\/2015\/452095","volume":"2015","author":"DA Hart","year":"2015","unstructured":"Hart, D. A. Curbing inflammation in multiple sclerosis and endometriosis: Should mast cells be targeted?. Int J Inflam. 2015, 452095. https:\/\/doi.org\/10.1155\/2015\/452095 (2015).","journal-title":"Int J Inflam."},{"key":"52888_CR40","doi-asserted-by":"publisher","first-page":"1468","DOI":"10.2106\/JBJS.I.00684","volume":"92","author":"MJ Monument","year":"2010","unstructured":"Monument, M. J. et al. The mast cell stabilizer ketotifen fumarate lessens contracture severity and myofibroblast hyperplasia: A study of a rabbit model of posttraumatic joint contractures. J. Bone Joint. Surg. Am. 92, 1468\u20131477. https:\/\/doi.org\/10.2106\/JBJS.I.00684 (2010).","journal-title":"J. Bone Joint. Surg. Am."},{"key":"52888_CR41","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1007\/s00011-011-0409-3","volume":"61","author":"MJ Monument","year":"2012","unstructured":"Monument, M. J. et al. The mast cell stabilizer ketotifen reduces joint capsule fibrosis in a rabbit model of post-traumatic joint contractures. Inflamm. Res. 61, 285\u2013292. https:\/\/doi.org\/10.1007\/s00011-011-0409-3 (2012).","journal-title":"Inflamm. Res."},{"key":"52888_CR42","doi-asserted-by":"publisher","first-page":"691830","DOI":"10.1155\/2013\/691830","volume":"2013","author":"NA Kamaruzaman","year":"2013","unstructured":"Kamaruzaman, N. A., Kardia, E., Kamaldin, N., Latahir, A. Z. & Yahaya, B. H. The rabbit as a model for studying lung disease and stem cell therapy. Biomed. Res. Int. 2013, 691830. https:\/\/doi.org\/10.1155\/2013\/691830 (2013).","journal-title":"Biomed. Res. Int."},{"key":"52888_CR43","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1007\/BF02074674","volume":"20","author":"M Ennis","year":"1987","unstructured":"Ennis, M., Lorenz, W., Gerland, W. & Heise, J. Isolation of mast cells from rabbit lung and liver: comparison of histamine release induced by the hypnotics Althesin and propanidid. Agents Actions. 20, 219\u2013222. https:\/\/doi.org\/10.1007\/BF02074674 (1987).","journal-title":"Agents Actions."},{"key":"52888_CR44","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20040981","author":"C Lanzi","year":"2019","unstructured":"Lanzi, C. et al. Role of histamine H(3) receptor antagonists on intraocular pressure reduction in rabbit models of transient ocular hypertension and glaucoma. Int. J. Mol. Sci. https:\/\/doi.org\/10.3390\/ijms20040981 (2019).","journal-title":"Int. J. Mol. Sci."},{"key":"52888_CR45","doi-asserted-by":"publisher","first-page":"1944","DOI":"10.1161\/CIRCRESAHA.121.318266","volume":"128","author":"BH Annex","year":"2021","unstructured":"Annex, B. H. & Cooke, J. P. New directions in therapeutic angiogenesis and arteriogenesis in peripheral arterial disease. Circ. Res. 128, 1944\u20131957. https:\/\/doi.org\/10.1161\/CIRCRESAHA.121.318266 (2021).","journal-title":"Circ. Res."},{"key":"52888_CR46","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.addr.2018.09.010","volume":"146","author":"AP Veith","year":"2019","unstructured":"Veith, A. P., Henderson, K., Spencer, A., Sligar, A. D. & Baker, A. B. Therapeutic strategies for enhancing angiogenesis in wound healing. Adv. Drug Deliv. Rev. 146, 97\u2013125. https:\/\/doi.org\/10.1016\/j.addr.2018.09.010 (2019).","journal-title":"Adv. Drug Deliv. Rev."},{"key":"52888_CR47","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1016\/S0002-9440(10)65582-4","volume":"153","author":"B Witzenbichler","year":"1998","unstructured":"Witzenbichler, B. et al. Vascular endothelial growth factor-C (VEGF-C\/VEGF-2) promotes angiogenesis in the setting of tissue ischemia. Am. J. Pathol. 153, 381\u2013394. https:\/\/doi.org\/10.1016\/S0002-9440(10)65582-4 (1998).","journal-title":"Am. J. Pathol."},{"key":"52888_CR48","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1038\/sj.gt.3301379","volume":"8","author":"Y Taniyama","year":"2001","unstructured":"Taniyama, Y. et al. Therapeutic angiogenesis induced by human hepatocyte growth factor gene in rat and rabbit hindlimb ischemia models: Preclinical study for treatment of peripheral arterial disease. Gene Ther. 8, 181\u2013189. https:\/\/doi.org\/10.1038\/sj.gt.3301379 (2001).","journal-title":"Gene Ther."},{"key":"52888_CR49","doi-asserted-by":"publisher","first-page":"628","DOI":"10.1006\/bbrc.1996.1556","volume":"227","author":"S Takeshita","year":"1996","unstructured":"Takeshita, S. et al. Therapeutic angiogenesis following arterial gene transfer of vascular endothelial growth factor in a rabbit model of hindlimb ischemia. Biochem. Biophys. Res. Commun. 227, 628\u2013635. https:\/\/doi.org\/10.1006\/bbrc.1996.1556 (1996).","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"52888_CR50","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1161\/01.cir.88.1.208","volume":"88","author":"LQ Pu","year":"1993","unstructured":"Pu, L. Q. et al. Enhanced revascularization of the ischemic limb by angiogenic therapy. Circulation 88, 208\u2013215. https:\/\/doi.org\/10.1161\/01.cir.88.1.208 (1993).","journal-title":"Circulation"},{"key":"52888_CR51","doi-asserted-by":"publisher","first-page":"1379","DOI":"10.1161\/01.hyp.33.6.1379","volume":"33","author":"R Morishita","year":"1999","unstructured":"Morishita, R. et al. Therapeutic angiogenesis induced by human recombinant hepatocyte growth factor in rabbit hind limb ischemia model as cytokine supplement therapy. Hypertension 33, 1379\u20131384. https:\/\/doi.org\/10.1161\/01.hyp.33.6.1379 (1999).","journal-title":"Hypertension"},{"key":"52888_CR52","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/j.mvr.2010.09.008","volume":"81","author":"M Li","year":"2011","unstructured":"Li, M. et al. Mutant hypoxia inducible factor-1alpha improves angiogenesis and tissue perfusion in ischemic rabbit skeletal muscle. Microvasc. Res. 81, 26\u201333. https:\/\/doi.org\/10.1016\/j.mvr.2010.09.008 (2011).","journal-title":"Microvasc. Res."},{"key":"52888_CR53","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1097\/FJC.0b013e3182982de7","volume":"62","author":"A Karatzas","year":"2013","unstructured":"Karatzas, A. et al. NGF promotes hemodynamic recovery in a rabbit hindlimb ischemic model through trkA- and VEGFR2-dependent pathways. J. Cardiovasc. Pharmacol. 62, 270\u2013277. https:\/\/doi.org\/10.1097\/FJC.0b013e3182982de7 (2013).","journal-title":"J. Cardiovasc. Pharmacol."},{"key":"52888_CR54","doi-asserted-by":"publisher","first-page":"886","DOI":"10.1016\/s0741-5214(98)70269-1","volume":"27","author":"SP Hopkins","year":"1998","unstructured":"Hopkins, S. P. et al. Controlled delivery of vascular endothelial growth factor promotes neovascularization and maintains limb function in a rabbit model of ischemia. J. Vasc. Surg. 27, 886\u2013894. https:\/\/doi.org\/10.1016\/s0741-5214(98)70269-1 (1998).","journal-title":"J. Vasc. Surg."},{"key":"52888_CR55","doi-asserted-by":"publisher","first-page":"3043","DOI":"10.1161\/01.cir.99.23.3043","volume":"99","author":"JE Fabre","year":"1999","unstructured":"Fabre, J. E., Rivard, A., Magner, M., Silver, M. & Isner, J. M. Tissue inhibition of angiotensin-converting enzyme activity stimulates angiogenesis in vivo. Circulation 99, 3043\u20133049. https:\/\/doi.org\/10.1161\/01.cir.99.23.3043 (1999).","journal-title":"Circulation"},{"key":"52888_CR56","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1016\/j.jconrel.2010.01.010","volume":"143","author":"YI Chung","year":"2010","unstructured":"Chung, Y. I. et al. Efficient revascularization by VEGF administration via heparin-functionalized nanoparticle-fibrin complex. J. Control Release. 143, 282\u2013289. https:\/\/doi.org\/10.1016\/j.jconrel.2010.01.010 (2010).","journal-title":"J. Control Release."},{"key":"52888_CR57","doi-asserted-by":"publisher","first-page":"201","DOI":"10.3892\/etm.2012.574","volume":"4","author":"N Nitta","year":"2012","unstructured":"Nitta, N. et al. Vascular regeneration by pinpoint delivery of growth factors using a microcatheter reservoir system in a rabbit hind-limb ischemia model. Exp. Ther. Med. 4, 201\u2013204. https:\/\/doi.org\/10.3892\/etm.2012.574 (2012).","journal-title":"Exp. Ther. Med."},{"key":"52888_CR58","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1007\/s00380-006-0934-0","volume":"22","author":"K Doi","year":"2007","unstructured":"Doi, K. et al. Enhanced angiogenesis by gelatin hydrogels incorporating basic fibroblast growth factor in rabbit model of hind limb ischemia. Heart Vessels 22, 104\u2013108. https:\/\/doi.org\/10.1007\/s00380-006-0934-0 (2007).","journal-title":"Heart Vessels"},{"key":"52888_CR59","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1159\/000139132","volume":"46","author":"R Wafai","year":"2009","unstructured":"Wafai, R., Tudor, E. M., Angus, J. A. & Wright, C. E. Vascular effects of FGF-2 and VEGF-B in rabbits with bilateral hind limb ischemia. J. Vasc. Res. 46, 45\u201354. https:\/\/doi.org\/10.1159\/000139132 (2009).","journal-title":"J. Vasc. Res."},{"key":"52888_CR60","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1080\/10623320390246432","volume":"10","author":"C Lebherz","year":"2003","unstructured":"Lebherz, C. et al. Therapeutic angiogenesis\/arteriogenesis in the chronic ischemic rabbit hindlimb: effect of venous basic fibroblast growth factor retroinfusion. Endothelium 10, 257\u2013265. https:\/\/doi.org\/10.1080\/10623320390246432 (2003).","journal-title":"Endothelium"},{"key":"52888_CR61","doi-asserted-by":"publisher","first-page":"W306","DOI":"10.2214\/AJR.07.4013","volume":"192","author":"A Seko","year":"2009","unstructured":"Seko, A. et al. Vascular regeneration by repeated infusions of basic fibroblast growth factor in a rabbit model of hind-limb ischemia. AJR Am. J. Roentgenol. 192, W306-310. https:\/\/doi.org\/10.2214\/AJR.07.4013 (2009).","journal-title":"AJR Am. J. Roentgenol."},{"key":"52888_CR62","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/j.cardiores.2003.10.003","volume":"61","author":"K Kondoh","year":"2004","unstructured":"Kondoh, K. et al. Conduction performance of collateral vessels induced by vascular endothelial growth factor or basic fibroblast growth factor. Cardiovasc. Res. 61, 132\u2013142. https:\/\/doi.org\/10.1016\/j.cardiores.2003.10.003 (2004).","journal-title":"Cardiovasc. Res."},{"key":"52888_CR63","doi-asserted-by":"publisher","first-page":"3322","DOI":"10.1161\/01.CIR.0000147779.17602.18","volume":"110","author":"A Hosaka","year":"2004","unstructured":"Hosaka, A. et al. Gelatin hydrogel microspheres enable pinpoint delivery of basic fibroblast growth factor for the development of functional collateral vessels. Circulation 110, 3322\u20133328. https:\/\/doi.org\/10.1161\/01.CIR.0000147779.17602.18 (2004).","journal-title":"Circulation"},{"key":"52888_CR64","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.mvr.2009.12.002","volume":"80","author":"J Li","year":"2010","unstructured":"Li, J. et al. Synergistic effects of FGF-2 and PDGF-BB on angiogenesis and muscle regeneration in rabbit hindlimb ischemia model. Microvasc. Res. 80, 10\u201317. https:\/\/doi.org\/10.1016\/j.mvr.2009.12.002 (2010).","journal-title":"Microvasc. Res."},{"key":"52888_CR65","doi-asserted-by":"publisher","first-page":"2053","DOI":"10.1016\/s0140-6736(02)08937-7","volume":"359","author":"RJ Lederman","year":"2002","unstructured":"Lederman, R. J. et al. Therapeutic angiogenesis with recombinant fibroblast growth factor-2 for intermittent claudication (the TRAFFIC study): A randomised trial. Lancet 359, 2053\u20132058. https:\/\/doi.org\/10.1016\/s0140-6736(02)08937-7 (2002).","journal-title":"Lancet"},{"key":"52888_CR66","doi-asserted-by":"publisher","first-page":"788","DOI":"10.1161\/hc0802.104407","volume":"105","author":"M Simons","year":"2002","unstructured":"Simons, M. et al. Pharmacological treatment of coronary artery disease with recombinant fibroblast growth factor-2: Double-blind, randomized, controlled clinical trial. Circulation 105, 788\u2013793. https:\/\/doi.org\/10.1161\/hc0802.104407 (2002).","journal-title":"Circulation"},{"key":"52888_CR67","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1161\/01.cir.102.5.565","volume":"102","author":"LH Gowdak","year":"2000","unstructured":"Gowdak, L. H. et al. Adenovirus-mediated VEGF(121) gene transfer stimulates angiogenesis in normoperfused skeletal muscle and preserves tissue perfusion after induction of ischemia. Circulation 102, 565\u2013571. https:\/\/doi.org\/10.1161\/01.cir.102.5.565 (2000).","journal-title":"Circulation"},{"key":"52888_CR68","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1038\/nm848","volume":"9","author":"R Cao","year":"2003","unstructured":"Cao, R. et al. Angiogenic synergism, vascular stability and improvement of hind-limb ischemia by a combination of PDGF-BB and FGF-2. Nat Med. 9, 604\u2013613. https:\/\/doi.org\/10.1038\/nm848 (2003).","journal-title":"Nat Med."},{"key":"52888_CR69","doi-asserted-by":"publisher","first-page":"662","DOI":"10.1172\/JCI117018","volume":"93","author":"S Takeshita","year":"1994","unstructured":"Takeshita, S. et al. Therapeutic angiogenesis. A single intraarterial bolus of vascular endothelial growth factor augments revascularization in a rabbit ischemic hind limb model. J Clin Invest. 93, 662\u2013670. https:\/\/doi.org\/10.1172\/JCI117018 (1994).","journal-title":"J Clin Invest."},{"key":"52888_CR70","doi-asserted-by":"publisher","first-page":"596170","DOI":"10.3389\/fcell.2020.596170","volume":"8","author":"K Chua","year":"2020","unstructured":"Chua, K. et al. Cord lining mesenchymal stem cells have a modest positive effect on angiogenesis in hindlimb ischemia. Front. Cell Dev. Biol. 8, 596170. https:\/\/doi.org\/10.3389\/fcell.2020.596170 (2020).","journal-title":"Front. Cell Dev. Biol."},{"key":"52888_CR71","doi-asserted-by":"publisher","first-page":"e67739","DOI":"10.1371\/journal.pone.0067739","volume":"8","author":"S Mikami","year":"2013","unstructured":"Mikami, S. et al. Autologous bone-marrow mesenchymal stem cell implantation and endothelial function in a rabbit ischemic limb model. PLoS One. 8, e67739. https:\/\/doi.org\/10.1371\/journal.pone.0067739 (2013).","journal-title":"PLoS One."},{"key":"52888_CR72","doi-asserted-by":"publisher","first-page":"2240","DOI":"10.1161\/CIRCULATIONAHA.109.856815","volume":"120","author":"MS Kim","year":"2009","unstructured":"Kim, M. S. et al. Priming with angiopoietin-1 augments the vasculogenic potential of the peripheral blood stem cells mobilized with granulocyte colony-stimulating factor through a novel Tie2\/Ets-1 pathway. Circulation 120, 2240\u20132250. https:\/\/doi.org\/10.1161\/CIRCULATIONAHA.109.856815 (2009).","journal-title":"Circulation"},{"key":"52888_CR73","doi-asserted-by":"publisher","first-page":"1069","DOI":"10.1111\/j.1745-7254.2005.00168.x","volume":"26","author":"CL Fan","year":"2005","unstructured":"Fan, C. L. et al. Therapeutic neovascularization by autologous transplantation with expanded endothelial progenitor cells from peripheral blood into ischemic hind limbs. Acta Pharmacol. Sin. 26, 1069\u20131075. https:\/\/doi.org\/10.1111\/j.1745-7254.2005.00168.x (2005).","journal-title":"Acta Pharmacol. Sin."},{"key":"52888_CR74","doi-asserted-by":"publisher","first-page":"1095","DOI":"10.3727\/096368911X623880","volume":"21","author":"EE Vaughan","year":"2012","unstructured":"Vaughan, E. E. et al. Pretreatment of endothelial progenitor cells with osteopontin enhances cell therapy for peripheral vascular disease. Cell Transpl. 21, 1095\u20131107. https:\/\/doi.org\/10.3727\/096368911X623880 (2012).","journal-title":"Cell Transpl."},{"key":"52888_CR75","doi-asserted-by":"publisher","first-page":"1442","DOI":"10.1038\/gt.2010.101","volume":"17","author":"WB Pyun","year":"2010","unstructured":"Pyun, W. B. et al. Naked DNA expressing two isoforms of hepatocyte growth factor induces collateral artery augmentation in a rabbit model of limb ischemia. Gene Ther. 17, 1442\u20131452. https:\/\/doi.org\/10.1038\/gt.2010.101 (2010).","journal-title":"Gene Ther."},{"key":"52888_CR76","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1161\/01.CIR.0000109700.81266.32","volume":"109","author":"N Tokunaga","year":"2004","unstructured":"Tokunaga, N. et al. Adrenomedullin gene transfer induces therapeutic angiogenesis in a rabbit model of chronic hind limb ischemia: Benefits of a novel nonviral vector, gelatin. Circulation 109, 526\u2013531. https:\/\/doi.org\/10.1161\/01.CIR.0000109700.81266.32 (2004).","journal-title":"Circulation"},{"key":"52888_CR77","doi-asserted-by":"publisher","first-page":"2255","DOI":"10.1161\/01.cir.102.18.2255","volume":"102","author":"KA Vincent","year":"2000","unstructured":"Vincent, K. A. et al. Angiogenesis is induced in a rabbit model of hindlimb ischemia by naked DNA encoding an HIF-1alpha\/VP16 hybrid transcription factor. Circulation 102, 2255\u20132261. https:\/\/doi.org\/10.1161\/01.cir.102.18.2255 (2000).","journal-title":"Circulation"},{"key":"52888_CR78","doi-asserted-by":"publisher","first-page":"2081","DOI":"10.1161\/01.cir.98.19.2081","volume":"98","author":"KG Shyu","year":"1998","unstructured":"Shyu, K. G., Manor, O., Magner, M., Yancopoulos, G. D. & Isner, J. M. Direct intramuscular injection of plasmid DNA encoding angiopoietin-1 but not angiopoietin-2 augments revascularization in the rabbit ischemic hindlimb. Circulation 98, 2081\u20132087. https:\/\/doi.org\/10.1161\/01.cir.98.19.2081 (1998).","journal-title":"Circulation"},{"key":"52888_CR79","doi-asserted-by":"publisher","first-page":"1465","DOI":"10.1161\/01.ATV.0000223865.64812.26","volume":"26","author":"K Kobayashi","year":"2006","unstructured":"Kobayashi, K. et al. Combination of in vivo angiopoietin-1 gene transfer and autologous bone marrow cell implantation for functional therapeutic angiogenesis. Arterioscler. Thromb. Vasc. Biol. 26, 1465\u20131472. https:\/\/doi.org\/10.1161\/01.ATV.0000223865.64812.26 (2006).","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"52888_CR80","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1089\/hum.2007.178","volume":"20","author":"O Pinkenburg","year":"2009","unstructured":"Pinkenburg, O. et al. Recombinant adeno-associated virus-based gene transfer of cathelicidin induces therapeutic neovascularization preferentially via potent collateral growth. Hum Gene Ther. 20, 159\u2013167. https:\/\/doi.org\/10.1089\/hum.2007.178 (2009).","journal-title":"Hum Gene Ther."},{"key":"52888_CR81","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1111\/j.1745-7254.2007.00527.x","volume":"28","author":"F Chen","year":"2007","unstructured":"Chen, F., Tan, Z., Dong, C. Y., Chen, X. & Guo, S. F. Adeno-associated virus vectors simultaneously encoding VEGF and angiopoietin-1 enhances neovascularization in ischemic rabbit hind-limbs. Acta Pharmacol. Sin. 28, 493\u2013502. https:\/\/doi.org\/10.1111\/j.1745-7254.2007.00527.x (2007).","journal-title":"Acta Pharmacol. Sin."},{"key":"52888_CR82","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1038\/aps.2010.67","volume":"31","author":"C Zhang","year":"2010","unstructured":"Zhang, C. et al. Angiopoiesis and bone regeneration via co-expression of the hVEGF and hBMP genes from an adeno-associated viral vector in vitro and in vivo. Acta Pharmacol. Sin. 31, 821\u2013830. https:\/\/doi.org\/10.1038\/aps.2010.67 (2010).","journal-title":"Acta Pharmacol. Sin."},{"key":"52888_CR83","doi-asserted-by":"publisher","first-page":"2467","DOI":"10.1161\/01.CIR.0000145139.53840.49","volume":"110","author":"Q Dai","year":"2004","unstructured":"Dai, Q. et al. Engineered zinc finger-activating vascular endothelial growth factor transcription factor plasmid DNA induces therapeutic angiogenesis in rabbits with hindlimb ischemia. Circulation 110, 2467\u20132475. https:\/\/doi.org\/10.1161\/01.CIR.0000145139.53840.49 (2004).","journal-title":"Circulation"},{"key":"52888_CR84","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.jss.2005.01.005","volume":"126","author":"H Jiang","year":"2005","unstructured":"Jiang, H., Zhang, T. & Sun, X. Vascular endothelial growth factor gene delivery by magnetic DNA nanospheres ameliorates limb ischemia in rabbits. J. Surg. Res. 126, 48\u201354. https:\/\/doi.org\/10.1016\/j.jss.2005.01.005 (2005).","journal-title":"J. Surg. Res."},{"key":"52888_CR85","doi-asserted-by":"publisher","first-page":"691","DOI":"10.3892\/etm.2014.1485","volume":"7","author":"K Cui","year":"2014","unstructured":"Cui, K. et al. Dual gene transfer of bFGF and PDGF in a single plasmid for the treatment of myocardial infarction. Exp. Ther. Med. 7, 691\u2013696. https:\/\/doi.org\/10.3892\/etm.2014.1485 (2014).","journal-title":"Exp. Ther. Med."},{"key":"52888_CR86","doi-asserted-by":"publisher","first-page":"837","DOI":"10.1038\/sj.gt.3301475","volume":"8","author":"N Ohara","year":"2001","unstructured":"Ohara, N. et al. Adenovirus-mediated ex vivo gene transfer of basic fibroblast growth factor promotes collateral development in a rabbit model of hind limb ischemia. Gene Ther. 8, 837\u2013845. https:\/\/doi.org\/10.1038\/sj.gt.3301475 (2001).","journal-title":"Gene Ther."},{"key":"52888_CR87","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.jss.2003.12.016","volume":"120","author":"S Nishikage","year":"2004","unstructured":"Nishikage, S. et al. In vivo electroporation enhances plasmid-based gene transfer of basic fibroblast growth factor for the treatment of ischemic limb. J. Surg. Res. 120, 37\u201346. https:\/\/doi.org\/10.1016\/j.jss.2003.12.016 (2004).","journal-title":"J. Surg. Res."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-52888-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-52888-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-52888-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T05:05:40Z","timestamp":1706763940000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-52888-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,29]]},"references-count":87,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["52888"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-52888-6","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.03.20.533550","asserted-by":"object"},{"id-type":"doi","id":"10.21203\/rs.3.rs-2997323\/v1","asserted-by":"object"}]},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,29]]},"assertion":[{"value":"29 May 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 January 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 January 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors (ET and ABB) have patented the technology presented in this manuscript. The other authors (MM, GH, JG, PF, LM, GC, ADS, and RS) on the study do not have competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"2352"}}