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In vitro, solutions were tested at pH 4.5\u00a0and\u00a05.5. The main in situ experiments were carried out at the pH of 4.5: For pellicle formation 6 volunteers wore bovine enamel slabs intraorally for 1\u00a0min, rinsed with 8\u00a0ml solution for 1\u00a0min and continued for up to 30\u00a0min\/8\u00a0h. Physiological pellicle samples served as controls. After incubation in HCl (2.0, 2.3) for 2\u00a0min mineral release was determined photometrically. Bacterial counts on 8\u00a0h biofilms were determined by fluorescence microscopy (BacLight\u2122 and DAPI with Concanavalin A). Modification of the pellicle ultrastructure was examined by TEM. Statistical analysis was performed using Kruskal\u2013Wallis and Mann\u2013Whitney-U tests with Bonferroni-correction (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). SnF<jats:sub>2<\/jats:sub> showed a significant erosion protection. AF, SnF<jats:sub>2<\/jats:sub>, and SnCl<jats:sub>2<\/jats:sub> were most anti-adherent. SnF<jats:sub>2<\/jats:sub> and SnCl<jats:sub>2<\/jats:sub> caused a pronounced basal pellicle with stannous precipitates. Compared to other fluoride monosubstances, stannous ions offer greater protection against erosive acidic attacks. Stannous ions act as crucial co-factor in this process.<\/jats:p>","DOI":"10.1038\/s41598-024-53732-7","type":"journal-article","created":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T17:02:23Z","timestamp":1707498143000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Impact of pH-adjusted fluoride and stannous solutions on the protective properties on the pellicle layer in vitro and in situ"],"prefix":"10.1038","volume":"14","author":[{"given":"N.","family":"Johannes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Hertel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Stoffel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Hannig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Basche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Schmitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Flemming","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Hannig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,9]]},"reference":[{"key":"53732_CR1","doi-asserted-by":"publisher","first-page":"cd007868","DOI":"10.1002\/14651858.CD007868.pub3","volume":"3","author":"T Walsh","year":"2019","unstructured":"Walsh, T., Worthington, H. 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