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To complete the life cycle, the parasite undergoes asexual and sexual reproduction within an intermediate snail host and a definitive mammalian host, respectively. The intra-molluscan phase provides a critical amplification step that ensures a successful transmission. However, the cellular and molecular mechanisms underlying the development of the intra-molluscan stages remain poorly understood. Here, single cell suspensions from\n                    <jats:italic>S. mansoni<\/jats:italic>\n                    mother sporocysts were\u00a0produced and sequenced using the droplet-based 10X Genomics Chromium platform. Six cell clusters comprising two tegument, muscle, neuron, parenchyma and stem\/germinal cell clusters were identified and validated by in situ hybridisation. Gene Ontology term analysis predicted key biological processes for each of the clusters, including three stem\/germinal sub-clusters. Furthermore, putative transcription factors predicted for stem\/germinal and tegument clusters may play key roles during parasite development and interaction with the intermediate host.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-55790-3","type":"journal-article","created":{"date-parts":[[2024,3,12]],"date-time":"2024-03-12T15:02:37Z","timestamp":1710255757000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Single-cell transcriptomics of the human parasite Schistosoma mansoni first intra-molluscan stage reveals tentative tegumental and stem-cell regulators"],"prefix":"10.1038","volume":"14","author":[{"given":"Carmen L.","family":"Diaz Soria","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Attenborough","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago","family":"Fontenla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennie","family":"Graham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Hall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sam","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toby G. R.","family":"Andrews","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate A.","family":"Rawlinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Berriman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Rinaldi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,12]]},"reference":[{"key":"55790_CR1","doi-asserted-by":"publisher","first-page":"e0009083","DOI":"10.1371\/journal.pntd.0009083","volume":"15","author":"DV Ayabina","year":"2021","unstructured":"Ayabina, D. V. et al. Gender-related differences in prevalence, intensity and associated risk factors of Schistosoma infections in Africa: A systematic review and meta-analysis. PLoS Negl. Trop. Dis. 15, e0009083. https:\/\/doi.org\/10.1371\/journal.pntd.0009083 (2021).","journal-title":"PLoS Negl. Trop. Dis."},{"key":"55790_CR2","doi-asserted-by":"publisher","first-page":"e327","DOI":"10.1016\/S1473-3099(22)00221-3","volume":"22","author":"NC Lo","year":"2022","unstructured":"Lo, N. C. et al. Review of 2022 WHO guidelines on the control and elimination of schistosomiasis. Lancet Infect. Dis. 22, e327\u2013e335. https:\/\/doi.org\/10.1016\/S1473-3099(22)00221-3 (2022).","journal-title":"Lancet Infect. Dis."},{"key":"55790_CR3","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02582-16","author":"N Vale","year":"2017","unstructured":"Vale, N. et al. Praziquantel for schistosomiasis: Single-drug metabolism revisited, mode of action, and resistance. Antimicrob. Agents Chemother. https:\/\/doi.org\/10.1128\/AAC.02582-16 (2017).","journal-title":"Antimicrob. Agents Chemother."},{"key":"55790_CR4","doi-asserted-by":"publisher","first-page":"790","DOI":"10.1016\/j.pt.2021.03.005","volume":"37","author":"D Nanes Sarfati","year":"2021","unstructured":"Nanes Sarfati, D., Li, P., Tarashansky, A. J. & Wang, B. Single-cell deconstruction of stem-cell-driven schistosome development. Trends Parasitol. 37, 790\u2013802. https:\/\/doi.org\/10.1016\/j.pt.2021.03.005 (2021).","journal-title":"Trends Parasitol."},{"key":"55790_CR5","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1038\/s41579-022-00810-0","volume":"20","author":"G Rinaldi","year":"2022","unstructured":"Rinaldi, G., Attenborough, T., Rawlinson, K. A. & Berriman, M. Schistosomes go single cell. Nat. Rev. Microbiol. 20, 705. https:\/\/doi.org\/10.1038\/s41579-022-00810-0 (2022).","journal-title":"Nat. Rev. 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Parasitology 64, 129\u2013141. https:\/\/doi.org\/10.1017\/s003118200004470x (1972).","journal-title":"Parasitology"},{"key":"55790_CR8","doi-asserted-by":"publisher","first-page":"935","DOI":"10.2307\/3278518","volume":"60","author":"PF Basch","year":"1974","unstructured":"Basch, P. F. & DiConza, J. J. The miracidium-sporocyst transition in Schistosoma mansoni: Surface changes in vitro with ultrastructural correlation. J. Parasitol. 60, 935\u2013941 (1974).","journal-title":"J. Parasitol."},{"key":"55790_CR9","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1007\/BF02462978","volume":"50","author":"M Koie","year":"1976","unstructured":"Koie, M. & Frandsen, F. Stereoscan observations of the miracidium and early sporocyst of Schistosoma mansoni. Z. Parasitenkd. 50, 335\u2013344. https:\/\/doi.org\/10.1007\/BF02462978 (1976).","journal-title":"Z. 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