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The aim of the present study was the investigation of the genomic features of two XDR <jats:italic>E. asburiae<\/jats:italic> ST229 clinical strains co-carrying <jats:italic>bla<\/jats:italic>NDM-1 and <jats:italic>bla<\/jats:italic>VIM-1 determinants, collected in October 2021 and in June 2022, respectively. Two <jats:italic>E. asburiae<\/jats:italic> strains were collected from rectal swabs of as many patients admitted to the cardiopulmonary intensive care unit of Fondazione I.R.C.C.S. \u201cPoliclinico San Matteo\u201d in Pavia, Italy. Based on the antibiotic susceptibility profile results, both isolates showed an XDR phenotype, retaining susceptibility only to fluoroquinolones. Both isolates shared identical resistome, virulome, plasmid content, and belonged to ST229, a rarely reported sequence type. They co-harbored <jats:italic>bla<\/jats:italic>NDM-1 and <jats:italic>bla<\/jats:italic>VIM-1 genes, that resulted located on transferable plasmids by conjugation and transformation. Moreover, both strains differed in 24 SNPs and showed genetic relatedness with <jats:italic>E. asburiae<\/jats:italic> ST709 and ST27. We described the first case of ST229 <jats:italic>E. asburiae<\/jats:italic> co-harboring <jats:italic>bla<\/jats:italic>NDM-1 and <jats:italic>bla<\/jats:italic>VIM-1 in Italy. This study points out the emergence of carbapenemases in low-risk pathogens, representing a novel challenge for public health, that should include such types of strains in dedicated surveillance programs. Antimicrobial susceptibility testing was carried out using Thermo Scientific\u2122 Sensititre\u2122 Gram Negative MIC Plates DKMGN. Both strains underwent whole-genome sequencing (WGS) using Illumina Miseq platform. Resistome, plasmidome, virulome, MLST, plasmid MLST and a SNPs-based phylogenetic tree were in silico determined.<\/jats:p>","DOI":"10.1038\/s41598-024-55884-y","type":"journal-article","created":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T16:02:47Z","timestamp":1710432167000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Enterobacter asburiae ST229: an emerging carbapenemases producer"],"prefix":"10.1038","volume":"14","author":[{"given":"Vittoria","family":"Mattioni Marchetti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Kuka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurora","family":"Piazza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Gaiarsa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Merla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariangela","family":"Sottosanti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Cambieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Migliavacca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fausto","family":"Baldanti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,14]]},"reference":[{"issue":"14","key":"55884_CR1","doi-asserted-by":"publisher","first-page":"e0047122","DOI":"10.1128\/aem.00471-22","volume":"88","author":"C Leister","year":"2022","unstructured":"Leister, C. & H\u00fcgler, M. 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