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The enrolled animals were designed as; twenty five apparently healthy buffalo\u2013cows considered as a control group and twenty five infected buffalo with endometritis. There were significant (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) increased of cervical diameter, endometrium thickness, uterine horn diameter, TAMEAN, TAMAX and blood flow through middle uterine artery with significant decrease of PI and RI values in endometritis buffalo\u2013cows. Gene expression levels were considerably higher in endometritis-affected buffaloes than in resistant ones for the genes <jats:italic>A2M<\/jats:italic>,<jats:italic> ADAMTS20<\/jats:italic>, <jats:italic>KCNT2<\/jats:italic>, <jats:italic>MAP3K4<\/jats:italic>, <jats:italic>MAPK14<\/jats:italic>, <jats:italic>FKBP5<\/jats:italic>, <jats:italic>FCAMR<\/jats:italic>, <jats:italic>TLR2<\/jats:italic>, <jats:italic>IRAK3<\/jats:italic>, <jats:italic>CCl2<\/jats:italic>, <jats:italic>EPHA4<\/jats:italic>, and <jats:italic>iNOS<\/jats:italic>. <jats:italic>The RXFP1<\/jats:italic>, <jats:italic>NDUFS5<\/jats:italic>, <jats:italic>TGF-\u03b2<\/jats:italic>, <jats:italic>SOD3<\/jats:italic>, <jats:italic>CAT<\/jats:italic>, and <jats:italic>GPX<\/jats:italic> genes were expressed at substantially lower levels in endometritis-affected buffaloes. The PCR-DNA sequence verdicts of healthy and affected buffaloes revealed differences in the SNPs in the amplified DNA bases related to endometritis for the investigated genes. However, <jats:italic>MAP3K4<\/jats:italic> elicited a monomorphic pattern. There was a significant decrease of red blood cells (RBCs) count, Hb and packed cell volume (PCV) with neutrophilia, lymphocytosis and monocytosis in endometritis group compared with healthy ones. The serum levels of Hp, SAA, Cp, IL-6, IL-10, TNF-\u03b1, NO and MDA were significantly (<jats:italic>P<\/jats:italic>\u02c20.05) increased, along with reduction of CAT, GPx, SOD and TAC in buffalo\u2013cows with endometritis compared to healthy ones. The variability of Doppler ultrasonographic scan and studied genes alongside alterations in the serum profile of investigated markers could be a reference guide for limiting buffalo endometritis through selective breeding of natural resistant animals.<\/jats:p>","DOI":"10.1038\/s41598-024-56258-0","type":"journal-article","created":{"date-parts":[[2024,3,8]],"date-time":"2024-03-08T08:02:05Z","timestamp":1709884925000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Doppler ultrasonographic scan, gene expression and serum profile of immune, APPs and antioxidant markers in Egyptian buffalo\u2013cows with clinical endometritis"],"prefix":"10.1038","volume":"14","author":[{"given":"Ahmed","family":"El-Sayed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Refaai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2851-1350","authenticated-orcid":false,"given":"Ahmed","family":"Ateya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,8]]},"reference":[{"key":"56258_CR1","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1016\/j.anireprosci.2010.08.022","volume":"124","author":"B Perera","year":"2011","unstructured":"Perera, B. 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