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Early and accurate detection of parasitic organisms is vital to saving lives. Deep learning models have uplifted the medical sector by providing promising results in diagnosing, detecting, and classifying diseases. This paper explores the role of deep learning techniques in detecting and classifying various parasitic organisms. The research works on a dataset consisting of 34,298 samples of parasites such as Toxoplasma Gondii, Trypanosome, Plasmodium, Leishmania, Babesia, and Trichomonad along with host cells like red blood cells and white blood cells. These images are initially converted from RGB to grayscale followed by the computation of morphological features such as perimeter, height, area, and width. Later, Otsu thresholding and watershed techniques are applied to differentiate foreground from background and create markers on the images for the identification of regions of interest. Deep transfer learning models such as VGG19, InceptionV3, ResNet50V2, ResNet152V2, EfficientNetB3, EfficientNetB0, MobileNetV2, Xception, DenseNet169, and a hybrid model, InceptionResNetV2, are employed. The parameters of these models are fine-tuned using three optimizers: SGD, RMSprop, and Adam. Experimental results reveal that when RMSprop is applied, VGG19, InceptionV3, and EfficientNetB0 achieve the highest accuracy of 99.1% with a loss of 0.09. Similarly, using the SGD optimizer, InceptionV3 performs exceptionally well, achieving the highest accuracy of 99.91% with a loss of 0.98. Finally, applying the Adam optimizer, InceptionResNetV2 excels, achieving the highest accuracy of 99.96% with a loss of 0.13, outperforming other optimizers. The findings of this research signify that using deep learning models coupled with image processing methods generates a highly accurate and efficient way to detect and classify parasitic organisms.<\/jats:p>","DOI":"10.1038\/s41598-024-56323-8","type":"journal-article","created":{"date-parts":[[2024,3,8]],"date-time":"2024-03-08T10:02:26Z","timestamp":1709892146000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Enhancing parasitic organism detection in microscopy images through deep learning and fine-tuned optimizer"],"prefix":"10.1038","volume":"14","author":[{"given":"Yogesh","family":"Kumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pertik","family":"Garg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manu Raj","family":"Moudgil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupinder","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcin","family":"Wo\u017aniak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Shafi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Fazal","family":"Ijaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,8]]},"reference":[{"issue":"1583","key":"56323_CR1","doi-asserted-by":"publisher","first-page":"3406","DOI":"10.1098\/rstb.2011.0092","volume":"366","author":"BL Hart","year":"2011","unstructured":"Hart, B. 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