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The majority of currently available anti-trichinellosis medications exhibit inadequate efficacy. The efficacy of a natively prepared new formulation of silver nanoparticles (Ag-NPs) was evaluated in the treatment of <jats:italic>Trichinella spiralis<\/jats:italic> (<jats:italic>T. spiralis<\/jats:italic>) infection in mice alone and combined with multivitamin-mineral (MM). After investigating the product\u2019s biological and pharmacological characteristics, its therapeutic dose was estimated to be Ag-NPs at 21.5\u00a0mg\/kg B.W. This dose was orally inoculated to experimentally infected mice at 3\u20135\u00a0days post-inoculation (dpi) against the mature worms, at 8\u201310\u00a0dpi against the newborn larvae, and at 33\u201335th\u00a0dpi against the encapsulated larvae. Each treatment\u2019s efficacy was assessed by scarifying control and treated mice 3\u00a0days post-treatment. The drug alone or in supplement form has a high trichinocidal effect exceeding that of the reference drug. Early treatment (3\u20135\u00a0dpi) by Ag-NPs or Ag-NPs\u2009+\u2009MM and albendazole revealed high efficacy against the intestinal stage, reaching 93.3%, 94.7%, and 90.6% for the three treatments, respectively. The materials causing a significant (<jats:italic>P<\/jats:italic>-value\u2009&lt;\u20090.001) decrease in the mean encapsulated larvae reached 86.61%, 89.07%, and 88.84%\/gm of muscles using the three treatments, respectively. Moreover, all larvae extracted from Ag-NPs-treated groups failed to induce infection post-inoculation in new mice. Additionally, combining the material with MM proved to overcome the reversible adverse effects of silver material on the estimated redox parameters and liver and kidney biomarkers, denoting its ability to alleviate Ag-NP toxicity. In conclusion, the high trichinocidal effect of Ag-NPs against the adult and encapsulated larvae during a short inoculation period introduced Ag-NPs as an alternative to other nematicidal drugs.<\/jats:p>","DOI":"10.1038\/s41598-024-56337-2","type":"journal-article","created":{"date-parts":[[2024,3,10]],"date-time":"2024-03-10T14:01:23Z","timestamp":1710079283000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Efficacy of silver nanoparticles against Trichinella spiralis in mice and the role of multivitamin in alleviating its toxicity"],"prefix":"10.1038","volume":"14","author":[{"given":"Noha Madbouly","family":"Taha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fady Sayed","family":"Youssef","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hend M.","family":"Auda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed M.","family":"El-Bahy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reem M.","family":"Ramadan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,10]]},"reference":[{"issue":"1\u20132","key":"56337_CR1","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.vetpar.2007.07.002","volume":"149","author":"E Pozio","year":"2007","unstructured":"Pozio, E. 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