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The biomarker of cell activation is found to be the 520\u00a0cm\n                    <jats:sup>\u22121<\/jats:sup>\n                    band, indicating formation of immunoglobulins. The blood samples are obtained from patients diagnosed with infectious mononucleosis and treated at the University Hospital in Krak\u00f3w. The lymphocytes\u2019 Raman spectra are collected using a mapping technique, exciting samples with a 514.5\u00a0nm line of Ar\n                    <jats:sup>\u2009+<\/jats:sup>\n                    \u2009laser. Measurements are performed on the 1st, 4th, 6th, 12th and 30th day of hospitalization, until the patient has recovered. The highest intensity of the immunoglobulin marker is observed on the 4th day of hospitalization, while the results of the blood count of patients show the greatest increase in the number of lymphocytes at the beginning of hospitalization. No activated lymphocytes were observed in the blood of healthy volunteers. Some information is provided by the evaluation of B-cell activation by estimating the activated areas in the cells, which are determined by the presence of the Ig marker. The 900\u00a0cm\n                    <jats:sup>\u22121<\/jats:sup>\n                    band and band around 1450\u00a0cm\n                    <jats:sup>\u22121<\/jats:sup>\n                    are also analyzed as markers of the presence of the latent membrane protein, LMP2A (and 2B), of the EBV viral protein. The anomalous degree of depolarization observed in B-cells in the course of EBV infection appears to be due to the influence of a virus protein, disrupting BCR signal transduction.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-56864-y","type":"journal-article","created":{"date-parts":[[2024,3,17]],"date-time":"2024-03-17T08:01:45Z","timestamp":1710662505000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Raman spectroscopy of lymphocytes from patients with the Epstein\u2013Barr virus infection"],"prefix":"10.1038","volume":"14","author":[{"given":"Magdalena","family":"Pietruszewska","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gra\u017cyna","family":"Biesiada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacek","family":"Czepiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malwina","family":"Birczy\u0144ska-Zych","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Moskal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksander","family":"Garlicki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Wese\u0142ucha-Birczy\u0144ska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,17]]},"reference":[{"key":"56864_CR1","doi-asserted-by":"publisher","first-page":"1343","DOI":"10.3390\/s7081343","volume":"7","author":"I Notingher","year":"2007","unstructured":"Notingher, I. 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