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Among the recently discovered barriers, the endogenous production of hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) via the reverse transsulfuration pathway, emerges as a noteworthy factor. In this study, we have explored the catalytic capabilities and crystal structure of cystathionine \u03b3-lyase from\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    (\n                    <jats:italic>Pa<\/jats:italic>\n                    CGL), a multidrug-opportunistic pathogen chiefly responsible for nosocomial infections. In addition to a canonical\n                    <jats:sc>l<\/jats:sc>\n                    -cystathionine hydrolysis,\n                    <jats:italic>Pa<\/jats:italic>\n                    CGL efficiently catalyzes the production of H\n                    <jats:sub>2<\/jats:sub>\n                    S using\n                    <jats:sc>l<\/jats:sc>\n                    -cysteine and\/or\n                    <jats:sc>l<\/jats:sc>\n                    -homocysteine as alternative substrates. Comparative analysis with the human enzyme and counterparts from other pathogens revealed distinct structural features within the primary enzyme cavities. Specifically, a distinctly folded entrance loop could potentially modulate the access of substrates and\/or inhibitors to the catalytic site. Our findings offer significant insights into the structural evolution of CGL enzymes across different pathogens and provide novel opportunities for developing specific inhibitors targeting\n                    <jats:italic>Pa<\/jats:italic>\n                    CGL.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-57625-7","type":"journal-article","created":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T14:03:04Z","timestamp":1713880984000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Catalytic specificity and crystal structure of cystathionine \u03b3-lyase from Pseudomonas aeruginosa"],"prefix":"10.1038","volume":"14","author":[{"given":"Marco","family":"Pedretti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Fern\u00e1ndez-Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Conter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iker","family":"Oyenarte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Favretto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adele","family":"di Matteo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Dominici","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Petrosino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Luz","family":"Martinez-Chantar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomas","family":"Majtan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Astegno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Alfonso","family":"Mart\u00ednez-Cruz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,23]]},"reference":[{"issue":"3","key":"57625_CR1","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/S1473-3099(17)30753-3","volume":"18","author":"E Tacconelli","year":"2018","unstructured":"Tacconelli, E. et al. 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