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This study investigated the changes in Young's modulus (YM), storage modulus (E\u2032), loss modulus (E\u02ba), and porosity of native and decellularized bovine reproductive tissues during the estrous cycle. Testis tunica albuginea had significantly higher YM, E\u2032, and E\u02ba than the inner testis, indicating greater stiffness and viscoelasticity. Endometrium showed no distinct differences in YM, E\u2032, or E\u02ba across the estrous cycle or between horns. Ovaries exhibited significant variations in YM, E\u2032, E\u02ba, and porosity, with higher YM and E\u2032 in the ipsilateral cortex and medulla during the luteal phase. Decellularized ovarian tissues displayed increased porosity. The oviduct displayed no significant differences in YM or E\u2032 in the isthmus, but the contralateral ampulla had reduced YM and E\u2032 in the luteal phase. 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