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In concordance with the current recommended WHO <jats:italic>pfhrp2<\/jats:italic> deletion surveillance strategy, 100 health facilities encompassing 10 regions of Tanzania enrolled malaria-suspected patients between February and July 2021. Of 7863 persons of all ages enrolled and providing RDT result and blood sample, 3777 (48.0%) were positive by the national RDT testing for <jats:italic>Plasmodium<\/jats:italic> lactate dehydrogenase (pLDH) and\/or HRP2. A second RDT testing specifically for the <jats:italic>P. falciparum<\/jats:italic> LDH (Pf-pLDH) antigen found 95 persons (2.5% of all RDT positives) were positive, though negative by the national RDT for HRP2, and were selected for <jats:italic>pfhrp2<\/jats:italic> and <jats:italic>pfhrp3<\/jats:italic> (<jats:italic>pfhrp2\/3<\/jats:italic>) genotyping. Multiplex antigen detection by laboratory bead assay found 135\/7847 (1.7%) of all blood samples positive for <jats:italic>Plasmodium<\/jats:italic> antigens but very low or no HRP2, and these were selected for genotyping as well. Of the samples selected for genotyping based on RDT or laboratory multiplex result, 158 were <jats:italic>P. falciparum<\/jats:italic> DNA positive, and 140 had sufficient DNA to be genotyped for <jats:italic>pfhrp2\/3<\/jats:italic>. Most of these (125\/140) were found to be <jats:italic>pfhrp2<\/jats:italic>+\/<jats:italic>pfhrp3<\/jats:italic>+, with smaller numbers deleted for only <jats:italic>pfhrp2<\/jats:italic> (n\u2009=\u20099) or only <jats:italic>pfhrp3<\/jats:italic> (n\u2009=\u20096). No dual <jats:italic>pfhrp2\/3<\/jats:italic> deleted parasites were observed. This survey found that parasites with these gene deletions are rare in Tanzania, and estimated that 0.24% (95% confidence interval: 0.08% to 0.39%) of false-negative HRP2-RDTs for symptomatic persons were due to <jats:italic>pfhrp2<\/jats:italic> deletions in this 2021 Tanzania survey. 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