{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:48:30Z","timestamp":1792806510825,"version":"4.5.0"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T00:00:00Z","timestamp":1712620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T00:00:00Z","timestamp":1712620800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Constipation is a widespread problem in paediatric practice, affecting almost 30% of children. One of the key causal factors of constipation may be disturbances in the homeostasis of the gastrointestinal microbiome. The aim of the study was to determine whether the oral and fecal microbiomes differ between children with and without constipation. A total of 91 children over three years of age were included in the study. Of these, 57 were qualified to a group with constipation, and 34 to a group without. The saliva and stool microbiomes were evaluated using 16S rRNA gene amplicon sequencing. Functional constipation was associated with characteristic bacterial taxa in the fecal microbiota. Statistically significant differences were found at the family level: Burkholderiaceae (q\u2009=\u20090.047), Christensenellaceae (q\u2009=\u20090.047), Chlostridiaceae (q\u2009=\u20090.047) were significantly less abundant in the constipation group, while the Tannerellaceae (q\u2009=\u20090.007) were more abundant. At the genus level, the significant differences were observed for rare genera, including <jats:italic>Christensenellaceae<\/jats:italic> r-7 (q\u2009=\u20092.88\u2009\u00d7\u200910<jats:sup>\u22122<\/jats:sup>), <jats:italic>Fusicatenibacter<\/jats:italic> (q\u2009=\u20092.88\u2009\u00d7\u200910<jats:sup>\u22122<\/jats:sup>), <jats:italic>Parabacteroides<\/jats:italic> (q\u2009=\u20091.63\u2009\u00d7\u200910<jats:sup>\u22122<\/jats:sup>), <jats:italic>Romboutsia<\/jats:italic> (q\u2009=\u20093.19\u2009\u00d7\u200910<jats:sup>\u22122<\/jats:sup>) and <jats:italic>Subdoligranulum<\/jats:italic> (q\u2009=\u20091.17\u2009\u00d7\u200910<jats:sup>\u22122<\/jats:sup>). All of them were less abundant in children with constipation. With the exception of significant taxonomic changes affecting only feces, no differences were found in the alpha and beta diversity of feces and saliva. Children with functional constipation demonstrated significant differences in the abundance of specific bacteria in the stool microbiome compared to healthy children. It is possible that the rare genera identified in our study which were less abundant in the constipated patients (<jats:italic>Christensellaceae r-7, Fusicatenibacter, Parabacteroides, Romboutsia<\/jats:italic> and <jats:italic>Subdoligranulum<\/jats:italic>) may play a role in protection against constipation. No significant differences were observed between the two groups with regard to the saliva microbiome.<\/jats:p>","DOI":"10.1038\/s41598-024-58642-2","type":"journal-article","created":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T09:03:01Z","timestamp":1712653381000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["The oral cavity and intestinal microbiome in children with functional constipation"],"prefix":"10.1038","volume":"14","author":[{"given":"Monika","family":"Kwiatkowska","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcin","family":"Go\u0142\u0119biewski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcin","family":"Sikora","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa \u0141o\u015b","family":"Rycharska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aneta","family":"Krogulska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,9]]},"reference":[{"key":"58642_CR1","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1152\/physiolgenomics.00082.2014","volume":"46","author":"L Zhu","year":"2014","unstructured":"Zhu, L. et al. Structural changes in the gut microbiome of constipated patients. Physiol. Genom. 46, 679\u2013686 (2014).","journal-title":"Physiol. Genom."},{"key":"58642_CR2","doi-asserted-by":"publisher","first-page":"153","DOI":"10.3389\/fcimb.2017.00153","volume":"7","author":"R Huang","year":"2017","unstructured":"Huang, R. & Hu, J. Positive effect of probiotics on constipation in children: A systematic review and meta-analysis of six randomized controlled trials. Front. Cell Infect. Microbiol. 7, 153 (2017).","journal-title":"Front. Cell Infect. Microbiol."},{"key":"58642_CR3","doi-asserted-by":"publisher","first-page":"e156","DOI":"10.1542\/peds.2009-1009","volume":"126","author":"MEJ Bongers","year":"2010","unstructured":"Bongers, M. E. J., van Wijk, M. P., Reitsma, J. B. & Benninga, M. A. Long-term prognosis for childhood constipation: Clinical outcomes in adulthood. Pediatrics 126, e156\u2013e162 (2010).","journal-title":"Pediatrics"},{"key":"58642_CR4","doi-asserted-by":"publisher","first-page":"6864","DOI":"10.3748\/wjg.v22.i30.6864","volume":"22","author":"S Rajindrajith","year":"2016","unstructured":"Rajindrajith, S., Devanarayana, N. M., Crispus Perera, B. J. & Benninga, M. A. Childhood constipation as an emerging public health problem. World J. Gastroenterol. 22, 6864 (2016).","journal-title":"World J. Gastroenterol."},{"key":"58642_CR5","doi-asserted-by":"publisher","first-page":"744","DOI":"10.1007\/s11427-017-9220-7","volume":"61","author":"L Huang","year":"2018","unstructured":"Huang, L., Zhu, Q., Qu, X. & Qin, H. Microbial treatment in chronic constipation. Sci. China Life Sci. 61, 744\u2013752 (2018).","journal-title":"Sci. China Life Sci."},{"key":"58642_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2016\/6787269","volume":"2016","author":"JG de Moraes","year":"2016","unstructured":"de Moraes, J. G., Motta, M. E. F. A., Beltr\u00e3o, M. F. S., Salviano, T. L. & da Silva, G. A. P. Fecal microbiota and diet of children with chronic constipation. Int. J. Pediatr. 2016, 1\u20138 (2016).","journal-title":"Int. J. Pediatr."},{"key":"58642_CR7","doi-asserted-by":"publisher","first-page":"e0164731","DOI":"10.1371\/journal.pone.0164731","volume":"11","author":"TGJ de Meij","year":"2016","unstructured":"de Meij, T. G. J. et al. Characterization of microbiota in children with chronic functional constipation. PLoS ONE 11, e0164731 (2016).","journal-title":"PLoS ONE"},{"key":"58642_CR8","doi-asserted-by":"publisher","first-page":"1378","DOI":"10.1111\/nmo.12628","volume":"27","author":"K Staller","year":"2015","unstructured":"Staller, K., Barshop, K., Kuo, B. & Ananthakrishnan, A. N. Resting anal pressure, not outlet obstruction or transit, predicts healthcare utilization in chronic constipation: A retrospective cohort analysis. Neurogastroenterol Motil 27, 1378\u20131388 (2015).","journal-title":"Neurogastroenterol Motil"},{"key":"58642_CR9","doi-asserted-by":"publisher","first-page":"397","DOI":"10.3949\/ccjm.84a.15141","volume":"84","author":"U Hayat","year":"2017","unstructured":"Hayat, U., Dugum, M. & Garg, S. Chronic constipation: Update on management. Cleve Clin. J. Med. 84, 397\u2013408 (2017).","journal-title":"Cleve Clin. J. Med."},{"key":"58642_CR10","doi-asserted-by":"publisher","first-page":"19","DOI":"10.3389\/fmed.2019.00019","volume":"6","author":"T Ohkusa","year":"2019","unstructured":"Ohkusa, T., Koido, S., Nishikawa, Y. & Sato, N. Gut microbiota and chronic constipation: A review and update. Front. Med. 6, 19 (2019).","journal-title":"Front. Med."},{"key":"58642_CR11","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1007\/s11894-019-0690-9","volume":"21","author":"MJ van Mill","year":"2019","unstructured":"van Mill, M. J., Koppen, I. J. N. & Benninga, M. A. Controversies in the management of functional constipation in children. Curr. Gastroenterol. Rep. 21, 23 (2019).","journal-title":"Curr. Gastroenterol. Rep."},{"key":"58642_CR12","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1053\/j.gastro.2015.10.005","volume":"150","author":"G Parthasarathy","year":"2016","unstructured":"Parthasarathy, G. et al. Relationship between microbiota of the colonic mucosa vs feces and symptoms, colonic transit, and methane production in female patients with chronic constipation. Gastroenterology 150, 367-379.e1 (2016).","journal-title":"Gastroenterology"},{"key":"58642_CR13","first-page":"1702","volume":"21","author":"V Ojetti","year":"2017","unstructured":"Ojetti, V. et al. Effect of Lactobacillus reuteri (DSM 17938) on methane production in patients affected by functional constipation: A retrospective study. Eur. Rev. Med. Pharmacol. Sci. 21, 1702\u20131708 (2017).","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"58642_CR14","first-page":"132","volume":"29","author":"MB Morais","year":"1999","unstructured":"Morais, M. B., V\u00edtolo, M. R., Aguirre, A. N. C. & Fagundes-Neto, U. measurement of low dietary fiber intake as a risk factor for chronic constipation in children. J. Pediatr. Gastroenterol. Nutr. 29, 132\u2013135 (1999).","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"58642_CR15","doi-asserted-by":"publisher","first-page":"838","DOI":"10.1016\/j.dld.2005.06.008","volume":"37","author":"I Khalif","year":"2005","unstructured":"Khalif, I., Quigley, E., Konovitch, E. & Maximova, I. Alterations in the colonic flora and intestinal permeability and evidence of immune activation in chronic constipation. Digest. Liver Dis. 37, 838\u2013849 (2005).","journal-title":"Digest. Liver Dis."},{"key":"58642_CR16","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1093\/ilar\/ilv017","volume":"56","author":"A Hiergeist","year":"2015","unstructured":"Hiergeist, A., Gl\u00e4sner, J., Reischl, U. & Gessner, A. Analyses of intestinal microbiota: culture versus sequencing: Figure 1. ILAR J 56, 228\u2013240 (2015).","journal-title":"ILAR J"},{"key":"58642_CR17","first-page":"e89","volume":"6","author":"A Tsuda","year":"2015","unstructured":"Tsuda, A. et al. Influence of proton-pump inhibitors on the luminal microbiota in the gastrointestinal tract: clinical and translational. Gastroenterology 6, e89 (2015).","journal-title":"Gastroenterology"},{"key":"58642_CR18","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1097\/MPG.0000000000000266","volume":"58","author":"MM Tabbers","year":"2014","unstructured":"Tabbers, M. M. et al. Evaluation and treatment of functional constipation in infants and children: Evidence-based recommendations from ESPGHAN and NASPGHAN. J. Pediatr. Gastroenterol. Nutr. 58, 265\u2013281 (2014).","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"58642_CR19","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1016\/S0016-5085(03)00888-6","volume":"125","author":"R van Ginkel","year":"2003","unstructured":"van Ginkel, R. et al. Childhood constipation: Longitudinal follow-up beyond puberty. Gastroenterology 125, 357\u2013363 (2003).","journal-title":"Gastroenterology"},{"key":"58642_CR20","doi-asserted-by":"publisher","first-page":"3916","DOI":"10.3748\/wjg.v17.i34.3916","volume":"17","author":"PV Guerra","year":"2011","unstructured":"Guerra, P. V. Pediatric functional constipation treatment with Bifidobacterium -containing yogurt: A crossover, double-blind, controlled trial. World J. Gastroenterol. 17, 3916 (2011).","journal-title":"World J. Gastroenterol."},{"key":"58642_CR21","doi-asserted-by":"publisher","first-page":"454","DOI":"10.5056\/jnm21130","volume":"28","author":"JL Kyung","year":"2022","unstructured":"Kyung, J. L. et al. Saccharomyces boulardii and Lactulose for childhood functional constipation: A multicenter randomized controlled trial. J. Neurogastroenterol. Motil. 28, 454\u2013462 (2022).","journal-title":"J. Neurogastroenterol. Motil."},{"key":"58642_CR22","doi-asserted-by":"publisher","first-page":"1682","DOI":"10.3390\/nu13051682","volume":"13","author":"E \u0141o\u015b-Rycharska","year":"2021","unstructured":"\u0141o\u015b-Rycharska, E. et al. A combined analysis of gut and skin microbiota in infants with food allergy and atopic dermatitis: A pilot study. Nutrients 13, 1682 (2021).","journal-title":"Nutrients"},{"key":"58642_CR23","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1007\/s00248-013-0344-7","volume":"67","author":"M Go\u0142\u0119biewski","year":"2014","unstructured":"Go\u0142\u0119biewski, M., Deja-Sikora, E., Cichosz, M., Tretyn, A. & Wr\u00f3bel, B. 16S rDNA pyrosequencing analysis of bacterial community in heavy metals polluted soils. Microb. Ecol. 67, 635\u2013647 (2014).","journal-title":"Microb. Ecol."},{"key":"58642_CR24","doi-asserted-by":"publisher","first-page":"180043","DOI":"10.1038\/sdata.2018.43","volume":"5","author":"A Callahan","year":"2018","unstructured":"Callahan, A., Winnenburg, R. & Shah, N. U-Index, a dataset and an impact metric for informatics tools and databases. Sci. Data 5, 180043 (2018).","journal-title":"Sci. Data"},{"issue":"11","key":"58642_CR25","doi-asserted-by":"publisher","first-page":"e1005752","DOI":"10.1371\/journal.pcbi.1005752","volume":"13","author":"F Rohart","year":"2017","unstructured":"Rohart, F., Gautiert, B., Singh, A. & Cao, K. mixOmics: An R package for omics feauture selection and multiple data integration. Plos Comput. Biol. 13(11), e1005752 (2017).","journal-title":"Plos Comput. Biol."},{"key":"58642_CR26","unstructured":"http:\/\/mixomics.org\/case-studies\/splsda-srbct-case-study"},{"key":"58642_CR27","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1038\/s41587-020-0548-6","volume":"38","author":"GM Douglas","year":"2020","unstructured":"Douglas, G. M. et al. PICRUSt2 for prediction of metagenome functions. Nat. Biotechnol. 38, 685\u2013688 (2020).","journal-title":"Nat. Biotechnol."},{"key":"58642_CR28","doi-asserted-by":"publisher","first-page":"836","DOI":"10.1111\/j.1651-2227.1998.tb01547.x","volume":"87","author":"G Zoppi","year":"2007","unstructured":"Zoppi, G. et al. The intestinal ecosystem in chronic functional constipation. Acta Paediatrica 87, 836\u2013841 (2007).","journal-title":"Acta Paediatrica"},{"key":"58642_CR29","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1016\/j.chom.2015.08.005","volume":"18","author":"F Teng","year":"2015","unstructured":"Teng, F. et al. Prediction of early childhood caries via spatial-temporal variations of oral microbiota. Cell Host Microbe 18, 296\u2013306 (2015).","journal-title":"Cell Host Microbe"},{"key":"58642_CR30","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1186\/s12866-016-0673-z","volume":"16","author":"Y Kim","year":"2016","unstructured":"Kim, Y. et al. Mucosal and salivary microbiota associated with recurrent aphthous stomatitis. BMC Microbiol 16, 57 (2016).","journal-title":"BMC Microbiol"},{"key":"58642_CR31","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.jaut.2017.03.009","volume":"83","author":"B Chen","year":"2017","unstructured":"Chen, B., Sun, L. & Zhang, X. Integration of microbiome and epigenome to decipher the pathogenesis of autoimmune diseases. J. Autoimmun. 83, 31\u201342 (2017).","journal-title":"J. Autoimmun."},{"key":"58642_CR32","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1038\/nm.3914","volume":"21","author":"X Zhang","year":"2015","unstructured":"Zhang, X. et al. The oral and gut microbiomes are perturbed in rheumatoid arthritis and partly normalized after treatment. Nat. Med. 21, 895\u2013905 (2015).","journal-title":"Nat. Med."},{"key":"58642_CR33","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.imlet.2004.02.005","volume":"93","author":"H Tlaskalov\u00e1-Hogenov\u00e1","year":"2004","unstructured":"Tlaskalov\u00e1-Hogenov\u00e1, H. et al. Commensal bacteria (normal microflora), mucosal immunity and chronic inflammatory and autoimmune diseases. Immunol. Lett. 93, 97\u2013108 (2004).","journal-title":"Immunol. Lett."},{"key":"58642_CR34","doi-asserted-by":"publisher","first-page":"201","DOI":"10.2334\/josnusd.16-0856","volume":"59","author":"Y Yamashita","year":"2017","unstructured":"Yamashita, Y. & Takeshita, T. The oral microbiome and human health. J. Oral Sci. 59, 201\u2013206 (2017).","journal-title":"J. Oral Sci."},{"key":"58642_CR35","doi-asserted-by":"publisher","first-page":"e34242","DOI":"10.1371\/journal.pone.0034242","volume":"7","author":"SM Huse","year":"2012","unstructured":"Huse, S. M., Ye, Y., Zhou, Y. & Fodor, A. A. A core human microbiome as viewed through 16S rRNA sequence clusters. PLoS ONE 7, e34242 (2012).","journal-title":"PLoS ONE"},{"key":"58642_CR36","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1128\/microbiolspec.GPP3-0042-2018","volume":"6","author":"J Abranches","year":"2018","unstructured":"Abranches, J. et al. Biology of Oral Streptococci. Microbiol Spectr 6, 10 (2018).","journal-title":"Microbiol Spectr"},{"key":"58642_CR37","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1186\/s12866-016-0898-x","volume":"16","author":"K Oki","year":"2016","unstructured":"Oki, K. et al. Comprehensive analysis of the fecal microbiota of healthy Japanese adults reveals a new bacterial lineage associated with a phenotype characterized by a high frequency of bowel movements and a lean body type. BMC Microbiol. 16, 284 (2016).","journal-title":"BMC Microbiol."},{"key":"58642_CR38","doi-asserted-by":"publisher","first-page":"433","DOI":"10.3390\/ijms20020433","volume":"20","author":"J Jalanka","year":"2019","unstructured":"Jalanka, J. et al. The effect of psyllium husk on intestinal microbiota in constipated patients and healthy controls. IJMS 20, 433 (2019).","journal-title":"IJMS"},{"key":"58642_CR39","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1097\/MCG.0000000000001107","volume":"54","author":"EB Hollister","year":"2020","unstructured":"Hollister, E. B. et al. Relationships of microbiome markers with extraintestinal, psychological distress and gastrointestinal symptoms, and quality of life in women with irritable bowel syndrome. J. Clin. Gastroenterol. 54, 175\u2013183 (2020).","journal-title":"J. Clin. Gastroenterol."},{"key":"58642_CR40","doi-asserted-by":"publisher","first-page":"121","DOI":"10.3920\/BM2018.0028","volume":"10","author":"AA Hibberd","year":"2019","unstructured":"Hibberd, A. A. et al. Probiotic or synbiotic alters the gut microbiota and metabolism in a randomised controlled trial of weight management in overweight adults. Benef. Microbes 10, 121\u2013135 (2019).","journal-title":"Benef. Microbes"},{"key":"58642_CR41","first-page":"191","volume":"11","author":"SJ Guzm\u00e1n-Casta\u00f1eda","year":"2018","unstructured":"Guzm\u00e1n-Casta\u00f1eda, S. J. et al. Gut microbiota composition explains more variance in the host cardiometabolic risk than genetic ancestry. Microbiology 11, 191 (2018).","journal-title":"Microbiology"},{"key":"58642_CR42","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1186\/s40168-018-0557-6","volume":"6","author":"Y He","year":"2018","unstructured":"He, Y. et al. Linking gut microbiota, metabolic syndrome and economic status based on a population-level analysis. Microbiome 6, 172 (2018).","journal-title":"Microbiome"},{"issue":"13","key":"58642_CR43","doi-asserted-by":"publisher","first-page":"2485","DOI":"10.3390\/foods12132485","volume":"12","author":"WS Ang","year":"2023","unstructured":"Ang, W. S. et al. A keystone gut bacterium christensenella minuta-A potential biotherapeutic agent for obesity and associated metabolic diseases. Foods 12(13), 2485 (2023).","journal-title":"Foods"},{"key":"58642_CR44","doi-asserted-by":"publisher","first-page":"1241259","DOI":"10.3389\/fmicb.2023.1241259","volume":"14","author":"O Ignatyeva","year":"2023","unstructured":"Ignatyeva, O. et al. Christensenella minuta, a new candidate next-generation probiotic: Current evidence and future trajectories. Front. Microbiol. Sec. Food Microbiol. 14, 1241259 (2023).","journal-title":"Front. Microbiol. Sec. Food Microbiol."},{"key":"58642_CR45","doi-asserted-by":"publisher","first-page":"1878","DOI":"10.1007\/s10620-006-9293-z","volume":"51","author":"S Delgado","year":"2006","unstructured":"Delgado, S., Su\u00e1rez, A. & Mayo, B. Bifidobacterial diversity determined by culturing and by 16S rDNA sequence analysis in feces and mucosa from ten healthy Spanish adults. Dig. Dis. Sci. 51, 1878\u20131885 (2006).","journal-title":"Dig. Dis. Sci."},{"key":"58642_CR46","doi-asserted-by":"crossref","unstructured":"EFSA Panel\u00a0on Biological Hazards (BIOHAZ), Koutsoumanis, K., Allende, A., Alvarez\u2010Ord\u00f3\u00f1ez, A., Bolton, D., Bover\u2010Cid, S., et al. Update of the list of QPS\u2010recommended biological agents intentionally added to food or feed as notified to EFSA 12: suitability of taxonomic units notified to EFSA until March 2020. EFS2 2020;18.","DOI":"10.2903\/j.efsa.2020.6174"},{"key":"58642_CR47","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1186\/s13099-020-00381-6","volume":"12","author":"PL Miller","year":"2020","unstructured":"Miller, P. L. & Carson, T. L. Mechanisms and microbial influences on CTLA-4 and PD-1-based immunotherapy in the treatment of cancer: A narrative review. Gut Pathog. 12, 43 (2020).","journal-title":"Gut Pathog."},{"key":"58642_CR48","doi-asserted-by":"publisher","first-page":"5561","DOI":"10.1038\/s41598-021-84964-6","volume":"11","author":"M Yadav","year":"2021","unstructured":"Yadav, M., Lomash, A., Kapoor, S., Pandey, R. & Chauhan, N. S. Mapping of the benzoate metabolism by human gut microbiome indicates food-derived metagenome evolution. Sci. Rep. 11, 5561 (2021).","journal-title":"Sci. Rep."},{"key":"58642_CR49","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1186\/s12903-018-0532-4","volume":"18","author":"T Tiwari","year":"2018","unstructured":"Tiwari, T., Mulvahill, M., Wilson, A., Rai, N. & Albino, J. Association between maternal acculturation and health beliefs related to oral health of Latino children. BMC Oral Health 18, 67 (2018).","journal-title":"BMC Oral Health"},{"key":"58642_CR50","doi-asserted-by":"publisher","first-page":"R42","DOI":"10.1186\/gb-2012-13-6-r42","volume":"13","author":"N Segata","year":"2012","unstructured":"Segata, N. et al. Composition of the adult digestive tract bacterial microbiome based on seven mouth surfaces, tonsils, throat and stool samples. Genome Biol. 13, R42 (2012).","journal-title":"Genome Biol."},{"key":"58642_CR51","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1038\/nature11053","volume":"486","author":"T Yatsunenko","year":"2012","unstructured":"Yatsunenko, T. et al. Human gut microbiome viewed across age and geography. Nature 486, 222\u2013227 (2012).","journal-title":"Nature"},{"key":"58642_CR52","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/imm.12765","volume":"152","author":"GLV de Oliveira","year":"2017","unstructured":"de Oliveira, G. L. V., Leite, A. Z., Higuchi, B. S., Gonzaga, M. I. & Mariano, V. S. Intestinal dysbiosis and probiotic applications in autoimmune diseases. Immunology 152, 1\u201312 (2017).","journal-title":"Immunology"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-58642-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-58642-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-58642-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T10:11:10Z","timestamp":1712657470000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-58642-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,9]]},"references-count":52,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["58642"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-58642-2","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,9]]},"assertion":[{"value":"8 January 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 April 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 April 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"8283"}}