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The purpose of this study was to determine the prevalence of pathogenic <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>E. coli<\/jats:italic>), the genetic linkages and routes of transmission between <jats:italic>E. coli<\/jats:italic> isolates from different animal species. The efficiency of disinfectants such as hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>), Virkon\u00aeS, TH<jats:sup>4+<\/jats:sup>, nano zinc oxide (ZnO NPs), and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-based zinc oxide nanoparticles (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\/ZnO NPs) against isolated strains of <jats:italic>E. coli<\/jats:italic> was evaluated. Using 100 fecal samples from different diarrheal species (cow n\u2009=\u200930, sheep n\u2009=\u200940, and broiler chicken n\u2009=\u200930) for <jats:italic>E. coli<\/jats:italic> isolation and identification using the entero-bacterial repetitive intergenic consensus (ERIC\u2013PCR) fingerprinting technique. The <jats:italic>E. coli<\/jats:italic> properties isolated from several diarrheal species were examined for their pathogenicity in vitro. Scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM), Fourier-transform infrared spectrum (FT-IR), X-ray diffraction (XRD), zeta potential, and particle size distribution were used for the synthesis and characterization of ZnO NPs and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\/ZnO NPs. The broth macro-dilution method was used to assess the effectiveness of disinfectants and disinfectant-based nanoparticles against <jats:italic>E. coli<\/jats:italic> strains<jats:italic>.<\/jats:italic> Regarding the results, the hemolytic activity and Congo red binding assays of pathogenic <jats:italic>E. coli<\/jats:italic> isolates were 55.3 and 44.7%, respectively. Eleven virulent <jats:italic>E. coli<\/jats:italic> isolates were typed into five ERIC-types (A1, A2, B1, B2, and B3) using the ERIC-PCR method. These types clustered into two main clusters (A and B) with 75% similarity. In conclusion, there was 90% similarity between the sheep samples' ERIC types A1 and A2. On the other hand, 89% of the ERIC types B1, B2, and B3 of cows and poultry samples were comparable. The H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\/ZnO NPs composite exhibits potential antibacterial action against <jats:italic>E. coli<\/jats:italic> isolates at 0.04\u00a0mg\/ml after 120\u00a0min of exposure.<\/jats:p>","DOI":"10.1038\/s41598-024-59280-4","type":"journal-article","created":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T02:02:00Z","timestamp":1713751320000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Efficacy evaluation of hydrogen peroxide disinfectant based zinc oxide nanoparticles against diarrhea causing Escherichia coli in ruminant animals and broiler chickens"],"prefix":"10.1038","volume":"14","author":[{"given":"Walaa I.","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7021-5520","authenticated-orcid":false,"given":"Asmaa N.","family":"Mohammed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AL-Shimaa A.","family":"Sleim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,22]]},"reference":[{"key":"59280_CR1","doi-asserted-by":"publisher","first-page":"693","DOI":"10.26873\/SVR-809-2019","volume":"56","author":"SA Allam","year":"2019","unstructured":"Allam, S. 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