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Recent studies report that these ticks also harbor human pathogens including <jats:italic>Borrelia burgdorferi<\/jats:italic> sensu lato, <jats:italic>Babesia microti<\/jats:italic>, and <jats:italic>Anaplasma phagocytophilum<\/jats:italic>. Therefore, studies that address the interactions of these ticks with human pathogens are important. In this study, we report the characterization of <jats:italic>H. longicornis<\/jats:italic> organic anion-transporting polypeptides (OATPs) in interactions of these ticks with <jats:italic>A. phagocytophilum<\/jats:italic>. Using OATP-signature sequence, we identified six OATPs in the <jats:italic>H. longicornis<\/jats:italic> genome. Bioinformatic analysis revealed that <jats:italic>H. longicornis<\/jats:italic> OATPs are closer to other tick orthologs rather than to mammalian counterparts. Quantitative real-time PCR analysis revealed that OATPs are highly expressed in immature stages when compared to mature stages of these ticks. In addition, we noted that the presence of <jats:italic>A. phagocytophilum<\/jats:italic> upregulates a specific OATP in these ticks. We also noted that exogenous treatment of <jats:italic>H. longicornis<\/jats:italic> with xanthurenic acid, a tryptophan metabolite, influenced OATP expression in these ticks. Immunoblotting analysis revealed that antibody generated against <jats:italic>Ixodes scapularis<\/jats:italic> OATP cross-reacted with <jats:italic>H. longicornis<\/jats:italic> OATP. Furthermore, treatment of <jats:italic>H. longicornis<\/jats:italic> with OATP antibody impaired colonization of <jats:italic>A. phagocytophilum<\/jats:italic> in these ticks. These results not only provide evidence that the OATP-tryptophan pathway is important for <jats:italic>A. phagocytophilum<\/jats:italic> survival in <jats:italic>H. longicornis<\/jats:italic> ticks but also indicate OATP as a promising candidate for the development of a universal anti-tick vaccine to target this bacterium and perhaps other rickettsial pathogens of medical importance.<\/jats:p>","DOI":"10.1038\/s41598-024-59315-w","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T20:29:09Z","timestamp":1713472149000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antibody-blocking of a tick transporter impairs Anaplasma phagocytophilum colonization in Haemaphysalis longicornis ticks"],"prefix":"10.1038","volume":"14","author":[{"given":"Prachi","family":"Namjoshi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald M.","family":"Lubembe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hameeda","family":"Sultana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Girish","family":"Neelakanta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,18]]},"reference":[{"key":"59315_CR1","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1016\/j.tig.2020.11.008","volume":"37","author":"N Jia","year":"2021","unstructured":"Jia, N. et al. 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