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A short anti-MAMP thiolated aptamer (Apt) with a methylene blue (MB) probe at 3\u02b9-end was immobilized on the surface of a gold electrode (MB-Apt-S\/GE). The electrochemical signal appeared when MAMP presenting in the sample solution competed with cDNA for binding with MB-Apt-S. Under optimized conditions, the liner range of this signal-on electrochemical aptasensor for the detection of MAMP achieved from 1.0 to 10.0\u00a0nmol\/L and 10.0\u2013400\u00a0nmol\/L. LOD 0.88\u00a0nmol\/L were obtained. Satisfactory spiked recoveries of saliva and urine were also obtained. In this method, only 5\u00a0min were needed to incubate before the square wave voltammetry (SWV) analysis, which was much more rapid than other electrochemical sensors, leading to a bright and broad prospect for the detection of MAMP in biological sample. This method can be used for on-site rapid detection on special occasions, such as drug driving scenes, entertainment venues suspected of drug use, etc.<\/jats:p>","DOI":"10.1038\/s41598-024-59505-6","type":"journal-article","created":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T00:01:45Z","timestamp":1713830505000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["An electrochemical aptasensor for methylamphetamine rapid detection by single-on mode based on competition with complementary DNA"],"prefix":"10.1038","volume":"14","author":[{"given":"Wenzhuo","family":"Chang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixiang","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjun","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongling","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuezhao","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunming","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,23]]},"reference":[{"key":"59505_CR1","doi-asserted-by":"publisher","first-page":"1814","DOI":"10.1016\/j.snb.2017.08.201","volume":"255","author":"Y Zhang","year":"2018","unstructured":"Zhang, Y. et al. 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