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Current sample preparation is limited by a tedious workflow requiring multiple steps, reagents and instrumentation, hampering nucleic acid testing at point of need. In this study, we present the use of mixed cellulose ester (MCE) paper for DNA binding by ionic interaction under molecular crowding conditions and fluid transport by wicking. The poly(ethylene) glycol-based (PEG) reagent simultaneously provides the high pH for alkaline lysis and crowding effects for ionic binding of the DNA under high salt conditions. In this study, we introduce Paper-based Abridged Solid-Phase Extraction with\u00a0Alkaline Poly(ethylene) Glycol Lysis (PASAP). The anionic mixed cellulose ester (MCE) paper\u00a0is used as solid phase and\u00a0allows for fluid transport by wicking, eliminating the need for pipetting skills and the use of a magnet to retain beads. Following the release of DNA from the cells due to the lytic activity of the PASAP solution, the DNA binds to the anionic surface of the MCE paper, concentrating at the bottom while the sample matrix is transported towards the top by wicking. The paper was washed by dipping it in 40% isopropanol for 10\u00a0s. After air-drying for 30\u00a0s, the bottom section of the paper (3\u2009mm\u00a0\u00d7\u20094\u00a0mm) was snapped off using the cap of a PCR tube and immersed in the colourimetric\u00a0loop-mediated isothermal amplification (cLAMP) solution for direct amplification and colourimetric detection. The total\u00a0sample processing was completed in 15 min and ready for amplification. cLAMP enabled the detection of 10<jats:sup>2<\/jats:sup> CFU\/mL of <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>E. coli<\/jats:italic>) from culture media and the detection of <jats:italic>E. coli<\/jats:italic> in milk\u2009&lt;\u200910<jats:sup>3<\/jats:sup>\u00a0CFU\/mL (10\u00a0CFU) after incubation at 68\u00a0\u00b0C for 60\u00a0min, demonstrating applicability of the method to complex biological samples.<\/jats:p>","DOI":"10.1038\/s41598-024-59763-4","type":"journal-article","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T11:03:19Z","timestamp":1719226999000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Method for lysis and paper-based elution-free DNA extraction with colourimetric isothermal amplification"],"prefix":"10.1038","volume":"14","author":[{"given":"Soo Min","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egan H.","family":"Doeven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosanne M.","family":"Guijt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,24]]},"reference":[{"key":"59763_CR1","doi-asserted-by":"publisher","first-page":"980","DOI":"10.3390\/bios13110980","volume":"13","author":"SM Lee","year":"2023","unstructured":"Lee, S. 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