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Conventional methods to detect antibiotics are time-consuming and require expensive specialized equipment. Here, we present a simple and rapid biosensor for detecting ampicillin, a commonly used antibiotic. Our method is based on the fluorescent properties of chitosan-coated Mn-doped ZnS micromaterials combined with the \u03b2-lactamase enzyme. The biosensors exhibited the highest sensitivity in a linear working range of 13.1\u201372.2\u00a0pM with a limit of detection of 8.24\u00a0pM in deionized water. In addition, due to the biological specificity of \u03b2-lactamase, the proposed sensors have demonstrated high selectivity over penicillin, tetracycline, and glucose through the enhancing and quenching effects at wavelengths of 510\u00a0nm and 614\u00a0nm, respectively. These proposed sensors also showed promising results when tested in various matrices, including tap water, bottled water, and milk. Our work reports for the first time the cost-effective (Mn:ZnS)Chitosan micromaterial was used for ampicillin detection. The results will facilitate the monitoring of antibiotics in clinical and environmental contexts.<\/jats:p>","DOI":"10.1038\/s41598-024-59772-3","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T08:02:13Z","timestamp":1714636933000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Dual-channel fluorescent sensors based on chitosan-coated Mn-doped ZnS micromaterials to detect ampicillin"],"prefix":"10.1038","volume":"14","author":[{"given":"Son Hai","family":"Nguyen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Van-Nhat","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai Thi","family":"Tran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,2]]},"reference":[{"key":"59772_CR1","doi-asserted-by":"publisher","first-page":"667","DOI":"10.3390\/antibiotics11050667","volume":"11","author":"MM Rahman","year":"2022","unstructured":"Rahman, M. 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