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Female calves were assigned to treatments: Creep (n\u2009=\u2009190), with ad libitum access to a nutritional supplement from 70 to 220\u00a0days after birth, or Control (n\u2009=\u2009140), without supplementation. After weaning (Day 220), both groups followed the same pasture and nutritional management. Body weight (BW) and backfat thickness (BFAT) were measured over time. Blood samples were collected at 220 and 360\u00a0days for LC\u2013MS\/MS targeted metabolomics. On day 408, during the synchronization timed artificial insemination (TAI) protocol, reproductive status (RS: diameter of uterine horn and largest follicle, and presence of CL) was assessed. Creep feeding increased BW and BFAT at weaning, but no differences in BW, BFAT, or RS after weaning\u00a0were observed. Nonetheless, the pregnancy per AI (P\/AI) for 1st service was 28.9% higher in the Creep group. On day 220, 11 significant metabolites influenced five metabolic pathways: Glucose-alanine cycle, alanine, glutathione, phenylalanine and tyrosine metabolism, and urea cycle. On day 360, 14 significant metabolites influenced eight metabolic pathways: Malate-aspartate shuttle, arginine and proline metabolism, urea cycle, aspartate, beta-alanine, glutamate metabolism, ammonia recycling and citric acid cycle. In conclusion, creep feeding supplementation improved calf performance and induced metabolic changes at weaning and 360\u00a0days of age. Although heifers had similar productive performance and reproductive status, when submitted to TAI, those supplemented with creep feeding had greater P\/AI.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-60216-1","type":"journal-article","created":{"date-parts":[[2024,4,27]],"date-time":"2024-04-27T07:01:53Z","timestamp":1714201313000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Metabolic imprinting in beef calves supplemented with creep feeding on performance, reproductive efficiency and metabolome profile"],"prefix":"10.1038","volume":"14","author":[{"given":"Bruna Lima Chechin","family":"Catussi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaqueline Rodrigues","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edson Guimar\u00e3es","family":"Lo Turco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio Carlos Franco","family":"Morgulis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro Sampaio","family":"Baruselli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,27]]},"reference":[{"key":"60216_CR1","doi-asserted-by":"publisher","first-page":"s210","DOI":"10.1017\/S1751731118001350","volume":"12","author":"B Dumont","year":"2018","unstructured":"Dumont, B., Groot, J. 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