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This study addresses this issue by modifying the chitosan with a sulfonamide derivative, 3-(4-(N,N-dimethylsulfonyl)phenyl)acrylic acid. The structure of the sulfonamide-chitosan derivative (DMS-CH) was confirmed using Fourier transform infrared spectroscopy and Nuclear magnetic resonance. The results of scanning electron microscopy, thermal gravimetric analysis, and X-ray diffraction indicated that the morphology changed to a porous nature, the thermal stability decreased, and the crystallinity increased in the DMS-CH derivative compared to chitosan, respectively. The degree of substitution was calculated from the elemental analysis data and was found to be moderate (42%). The modified chitosan exhibited enhanced antimicrobial properties at low concentrations, with a minimum inhibitory concentration (MIC) of 50 \u00b5g\/mL observed for <jats:italic>B. subtilis<\/jats:italic> and <jats:italic>P. aeruginosa<\/jats:italic>, and a value of 25 \u00b5g\/mL for <jats:italic>S. aureus<\/jats:italic>, <jats:italic>E. coli<\/jats:italic>, and <jats:italic>C. albicans<\/jats:italic>. In the case of native chitosan, the MIC values doubled or more, with 50 \u00b5g\/mL recorded for <jats:italic>E. coli<\/jats:italic> and <jats:italic>C. albicans<\/jats:italic> and 100 \u03bcg\/mL recorded for <jats:italic>B. subtilis<\/jats:italic>, <jats:italic>S. aureus<\/jats:italic>, and <jats:italic>P. aeruginosa<\/jats:italic>. Furthermore, toxicological examinations conducted on MCF-7 (breast adenocarcinoma) cell lines demonstrated that DMS-CH exhibited greater toxicity (IC50\u2009=\u2009225.47 \u03bcg\/mL) than pure CH, while still maintaining significant safety limits against normal lung fibroblasts (WI-38). Collectively, these results suggest the potential use of the newly modified chitosan in biomedical applications.<\/jats:p>","DOI":"10.1038\/s41598-024-60456-1","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T16:02:31Z","timestamp":1714752151000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Fabrication and characterization of a new eco-friendly sulfonamide-chitosan derivative with enhanced antimicrobial and selective cytotoxicity properties"],"prefix":"10.1038","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0460-734X","authenticated-orcid":false,"given":"Ahmed G.","family":"Ibrahim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed G.","family":"Hamodin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amr","family":"Fouda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed M.","family":"Eid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walid E.","family":"Elgammal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,3]]},"reference":[{"key":"60456_CR1","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1016\/j.jhazmat.2014.05.061","volume":"276","author":"Z Yang","year":"2014","unstructured":"Yang, Z. et al. 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